One-stage versus two-stage piezocision-assisted orthodontic tooth movement: A preclinical study based on Nano-CT and RT-PCR analyses - 17/03/22

Doi : 10.1016/j.jormas.2022.03.010

Carole Charavet ^a,^b,^c,^d,^⁎ , Dorien Van Hede ^d,^e, Sandy Anania ^d,^f, Nathalie Maes ^g, Adelin Albert ^h, France Lambert ^d,^e
^a Université Côte d'Azur, Faculté de Chirurgie-Dentaire, Département d'Orthodontie, Nice, France
^b CHU de Nice, Pôle Odontologie, UEC d'Orthodontie, Nice, France
^c Laboratoire Microbiologie orAle Immunité Santé MICORALIS (UPR 7354), Université Côted'Azur, France
^d Dental Biomaterials Research Unit, University of Liège, Belgium
^e Department of Periodontology, Oral Surgery and Implant Surgery, University Hospital of Liège, Liège, Belgium
^f Metastasis Research Laboratory (MRL), GIGA-Cancer, Pathology Institute B23, University of Liège, Liège, Belgium
^g Biostatistics and Medico-Economic Information Department, University Hospital of Liège, Liège, Belgium
^h Department of Public Health, University of Liège, Liège, Belgium

^⁎Corresponding author at: Faculté de Chirurgie-Dentaire, Département d'Orthodontie, Université Côte d'Azur, 06300 Nice, France.UFR OdontologieDépartement d'OrthodontieUniversité Côte d'Azur5, Avenue du 22ième BCANice06357France

En prensa. Pruebas corregidas por el autor. Disponible en línea desde el Thursday 17 March 2022
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Abstract

Objective

To evaluate the effect of a second-stage piezocision on the biological response.

Materials and methods

60 rats were randomly allocated to 6 experimental groups of 10 rats. Rats undergoing a one-stage piezocision were sacrified on day 7, 28 and 42 (groups 1–3) while rats undergoing a two-satge piezocision were sacrified on day 42, 63 and 90 (groups 4–6), respectively. The biological response was investigated in 3D at the tissue level using Nano-computed tomography (Nano-CT) and, at the molecular level using the qRT-PCR technique. Bone Volume Fraction (BVF) loss was the primary endpoint.

Results

Similar loss of BVF were observed both after the first and second piezocisions. The change in BVF loss between 7 and 28 days after each piezocision were 25.1 ± 13.0 (SE)% and 11.2 ± 11.6 (SE)% respectively and did not differ from each other (p = 0.43). Changes in BVF loss from 7 to 42 days were also comparable in one-stage and two-stage piezocision (4.9 ± 12.3 (SE) vs. -19.9 ± 13.4 (SE), p = 0.19). At the molecular level, all parameters except Translating Ribosome Affinity Purification (TRAP) protein had identical patterns.

Conclusion

Within the limits of the present study, a second piezocision allowed to re-induce the Regional Acceleratory Phenomenon (RAP) effect. Nevertheless, the relevance of the findings to the clinical effect has not been tested.

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Keywords : Piezocision, Piezosurgery, Corticotomy, Accelerated orthodontics, Regional acceleratory phenomenon