Now, accepted treatments for Female Sexual Dysfunction (FSD) are limited to surgical, psychological, behavioral, hormonal and psychopharmacologic interventions. Because of the complex and multifactorial nature of FSD, current therapeutic options may leave a subset of women suffering with sexual dysfunction without clinical improvement and may be with side effects of the procedures. This is a study to evaluate the effect of platelet rich plasma (PRP) in treating FSD FGM/C. PRP was administered to the anterior vaginal wall (1 session), clitoris of 45 females with FGM/C and non-FGM/C with sexual dysfunction and orgasmic disorder [Female Sexual Function Index (FSFI) total score≤26 orgasmic subdomain score≤3.75]. The correlation between PRP sessions and FSFI domain scores was done with Pearson's correlation and linear regression analysis. Following the application of the PRP, the total FSFI score was converted significantly in both FGM/C and non-FGM/C (P<0.05). A significant change was observed in all sub-domains of FSFI score after PRP (P<0.05). The total FSFI score increased in all 45 females [16 non-FGM/C and 29 FGM/C] (P<0.05). That correlations between all sub-domains of FSFI score before and after PRP injection were significantly positive (P<0.05). Also, there were statistically significant positive correlations between total FSFI scores before and after PRP injection, and sociodemographic and obstetric characteristics of the study population in both FGM/C and non-FGM/C. As a minimally invasive method, PRP administration to the distal anterior vaginal wall may improve female sexuality with high satisfaction in FGM/C.