Multilayered microcapsules for the sustained-release of angiogenic proteins from encapsulated cells - 20/08/11
Corresponding author: Tel.: 312-567-5098; fax: 312-567-5770Abstract |
Background |
Multilayered alginate microcapsules with a permselective poly-L-ornithine membrane can be used for the dual purpose of encapsulating cells in the inner core and sustained release of angiogenic proteins from the outer layer. The aim of this study was to examine the encapsulation and release of a novel chimeric form of fibroblast growth factor–1 (FGF-1) from the outer layer of alginate microcapsules.
Methods |
Heparin-binding growth-associated molecule bound to FGF-1 (HB-GAM/FGF-1) was encapsulated in the outer layer of multilayered alginate microbeads constructed using varying alginate conditions. The encapsulation and release of the chimera was quantified.
Results |
The outer layer was able to encapsulate and release HB-GAM/FGF-1 for up to 30 days. The outer layer made with 1% alginate of high mannuronic acid content provided the fastest release, while 1.25% high guluronic acid content alginate displayed the longest duration of release.
Conclusions |
The outer layer of multilayered alginate microbeads can be used for the encapsulation and long-term release of HB-GAM/FGF-1.
El texto completo de este artículo está disponible en PDF.Keywords : Alginate, Neovascularization, FGF-1, Heparin-binding growth-associated molecule
Esquema
| This study was supported by grants 0852048, 0731201, and 0854430 from the National Science Foundation (Arlington, VA), by the US Department of Veterans Affairs (Washington, DC) (E.M.B., H.P.G.), and by grant RO 1 DK080897 from the National Institutes of Health (Bethesda, MD). Mr Khanna received support from a generous donation by Mr Edward Ross and Dr Monica Moya from the Bill & Melinda Gates Foundation (Seattle, WA). |
Vol 200 - N° 5
P. 655-658 - novembre 2010 Regresar al número
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