Hericium erinaceus extracts promote neuronal differentiation and excitability through nootropic metabolite activity - 14/06/25
Abstract |
This study investigates the effects of a blend of Hericium erinaceus (lion's mane mushroom) extract on the differentiation of SH-SY5Y cells, a human neuroblastoma cell line, revealing potential therapeutic implications for neuroblastoma management. Treatment with this blend induced cells differentiation towards a neuron-like profile, as evidenced by enhanced neuronal excitability and upregulation of neuronal markers, such as βIII-tubulin and synaptotagmin. Additionally, the treatment significantly reduced PCNA, a key regulator of proliferation, alongside a decrease in stemness markers, indicating a shift toward a more mature and less proliferative phenotype. These findings demonstrate the ability of Hericium erinaceus to promote neuronal differentiation and inhibit proliferation in neuroblastoma cells, highlighting its therapeutic potential for managing neuroblastoma and potentially other neurological disorders. The results suggest that Hericium erinaceus may serve as a promising candidate for the development of novel neuroregenerative therapies.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Schematic summary of the effects of Hericium erinaceus (He) extracts, containing bioactive metabolites such as erinacines, hericenones, and ergothioneine, on SH-SY5Y neuroblastoma cells. Bioactive compounds derived from both the sporophore and mycelium promote neuronal differentiation—evidenced by increased neurite length, β-tubulin expression, synaptotagmin levels, and excitability—while simultaneously reducing markers of proliferation including PCNA, CD133, and clonogenic potential.
Schematic summary of the effects of Hericium erinaceus (He) extracts, containing bioactive metabolites such as erinacines, hericenones, and ergothioneine, on SH-SY5Y neuroblastoma cells. Bioactive compounds derived from both the sporophore and mycelium promote neuronal differentiation—evidenced by increased neurite length, β-tubulin expression, synaptotagmin levels, and excitability—while simultaneously reducing markers of proliferation including PCNA, CD133, and clonogenic potential.Le texte complet de cet article est disponible en PDF.
Highlights |
• | H. erinaceus extract induces neuronal differentiation in SH-SY5Y cells. |
• | Treatment enhances voltage-gated sodium currents and AP firing properties. |
• | Neuronal marker expression increases while proliferation markers decrease. |
• | Nav channel upregulation confirmed by patch clamp and immunofluorescence. |
• | LUHMES neuron model confirms lack of excitotoxicity in healthy cells. |
Abbreviations : TPA 12-O, He, MTT assay 3, Cm, Rm, Vm, TTX, VGSC, I-V plots, SNARE, PCNA
Keywords : Neuroblastoma, SH-SY5Y, Hericium erinaceus, Neuronal differentiation, Patch clamp, Voltage-gated sodium currents, Cell proliferation, Action potential, Erinacines, Hericenones, Voltage-Gated Sodium Channel
Plan
Vol 188
Article 118204- juillet 2025 Retour au numéro
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