Gastroprotective effect and mechanism of action of Croton rhamnifolioides essential oil in mice - 25/04/17
, Anita Oliveira Brito Pereira Bezerra Martins a, e , Andressa de Alencar Silva a, e , Maria Rayane Correia de Oliveira a, e , Jaime Ribeiro-Filho b, e , Thaís Rodrigues de Albuquerque a, e , Henrique Douglas Melo Coutinho c, e , Jackson Roberto Guedes da Silva Almeida d, e , Lucindo Jose Quintans d, e , Irwin Rose Alencar de Menezes a, e, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 4 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Abstract |
Background |
Croton rhamnifolioides Pax is a plant species that have been used in the folk medicine to treat ulcers, inflammations and hypertension. However, despite the relevant data obtained from ethnopharmacological studies, the pharmacological properties endorsing the efficacy of this plant to treat ulcer remain to be elucidated.
Hypothesis/Purpose |
The present study aimed to characterize the chemical profile and evaluate the gastroprotective activity of the essential oil obtained from C. rhamnifolioides Pax (OECC) in mice.
Methods |
The essential oil of Croton rhamnifolioides was obtained by hydrodistillation and analyzed by gas-phase chromatography coupled to mass spectrometry (GC/MS). The median lethal dose was determined employing an acute toxicity test. The gastroprotective activity of the OECC was investigated using animal models of gastric ulcer induced by the administration of absolute ethanol, acidified ethanol or indomethacin. Mechanisms of action were investigated using the physical barrier test and by in vivo evaluation of the involvement of the following molecular pathways: nitric oxide, ATP – dependent potassium channels, α2 – noradrenergic receptors, capsaicin – sensitive afferent neurons and opioid receptor.
Results |
We identified the presence of 21 compounds in OECC, including spathulenol and 1,8 – cineole as major constituents. In orally administered mice, OECC caused no significant toxicity. OECC significantly prevented gastric lesions in all mice models. The barrier test demonstrated that the gastroprotective activity of OECC occurs in a systemic dimension. Our results demonstrated that the gastroprotective effect of OECC involves mechanisms that are related to modulation of opioid receptors and nitric oxide.
Conclusion |
In conclusion, OECC demonstrated significant gastroprotective activity associated with low toxicity, providing scientific evidences that C. rhamnifolioides have the potential for the development of new antiulcer drugs.
Le texte complet de cet article est disponible en PDF.Abbreviations : OECC, PGE2, NO, GPCRs, ICMBio, CONCEA, NSAID, DZO, LNAME, TRPV1, ACh, PLC, H2, cAMP, GC/MS, GIT
Keywords : Croton rhamnifolioides, Essential oil, Gastroprotection, Mechanism of action, Chemical profile
Plan
Vol 89
P. 47-55 - mai 2017 Retour au numéro
Article précédent
- Enhanced anticancer effect of Combretastatin A-4 phosphate when combined with vincristine in the treatment of hepatocellular carcinoma
- Esam M. Aboubakr, Ashraf Taye, Omar M. Aly, Amira M. Gamal-Eldeen, Mohamed A. El-Moselhy
- H2S causes contraction and relaxation of major arteries of the rabbit
- Martin Caprnda, Tawar Qaradakhi, Joanne L Hart, Nazarii Kobyliak, Radka Opatrilova, Peter Kruzliak, Anthony Zulli
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?