PSMB8 regulates glioma cell migration, proliferation, and apoptosis through modulating ERK1/2 and PI3K/AKT signaling pathways - 09/03/18
, Yan-song Zhang eBienvenue sur EM-consulte, la référence des professionnels de santé.
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Highlights |
• | The results indicated that knockdown of PSMB8 can inhibit the proliferation and migration of glioma cells by reducing expression of cyclin D1, cyclin A, cyclin B1, and Vimentin, N-cadherin and increasing E-cadherin expression via the ERK1/2 and PI3k/AKT pathways in glioma. Besides, PSMB8 can induce apoptosis of human glioma cell lines. |
• | Downregulation of PSMB8 may be a useful strategy for the treatment of PSMB8-overexpressing cancers. |
Abstract |
Glioma has been considered as one of the most aggressive and popular brain tumors of patients. It is essential to explore the mechanism of glioma. In this study, we established PSMB8 as a therapeutic target for glioma treatment. Expression of PSMB8 as well as Ki-67 was higher in glioma tissues demonstrated by western blot and immunohistochemistry. Then, the role of PSMB8 in migration and proliferation of glioma cells was investigated by conducting wound-healing, trans-well assay, cell counting kit (CCK)-8, flow cytometry assay and colony formation analysis. The data showed that interfering PSMB8 may inhibit the migration and proliferation of glioma cells by reducing expression of cyclin A, cyclin B1, cyclin D1, Vimentin, and N-cadherin, and by increasing expression of E-cadherin. Additionally, interfering PSMB8 may induce apoptosis of glioma cells by upregulating caspase-3 expression. Furthermore, these in vitro findings were validated in vivo and the ERK1/2 and PI3k/AKT signaling pathways were involved in PSMB8-triggered migration and proliferation of glioma cells. In an in vivo model, downregulation of PSMB8 suppressed tumor growth. In conclusion, PSMB8 is closely associated with migration, proliferation, and apoptosis of glioma cells, and might be considered as a novel prognostic indicator in patients with gliomas.
Le texte complet de cet article est disponible en PDF.Keywords : PSMB8, Glioma, AKT, ERK1/2
Plan
Vol 100
P. 205-212 - avril 2018 Retour au numéro
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