Effect of HIF-1α inhibitors for preventing the progress of muscle contracture in rat soleus muscles - 15/07/18
Résumé |
Introduction/Background |
Immobilization-induced muscle fibrosis is important regarding the pathogenesis of muscle contracture, and the therapeutic target for this fibrotic lesion is unknown. On the other hand, hypoxia-inducible factor (HIF)-1α is expected to be a new therapeutic target for fibrotic disease in the viscera. Accordingly, preventing the negative alteration of HIF-1α may suppress the progress of immobilization-induced muscle fibrosis. Therefore, this study investigated the effect of the HIF-1α inhibitor for immobilization-induced muscle fibrosis in rat soleus muscles.
Material and method |
Wistar rats were randomly divided into control and experimental groups. In the experimental group, both ankle joints were fixed in full plantar flexion with plaster casts for 4 weeks. Some rats in the experimental group were immobilized throughout the 4-week period (immobilization group), whereas other rats in the experimental group were treated with YC-1, an HIF-1α inhibitor (YC-1 group). Additionally, YC-1 (2mg/mL/day) was administered to each rat for 4 weeks. Bilateral dorsiflexion range of motion (ROM) in the ankle joints was measured each week to evaluate muscle contracture. Moreover, hydroxyproline (a parameter for collagen contents) and protein levels of HIF-1α were determined via biochemical analysis.
Results |
Dorsiflexion ROM was significantly smaller in both experimental groups than in the control group at each timepoint, whereas this parameter was significantly greater in the YC-1 group than in the immobilization group after 3 weeks. In addition, the hydroxyproline and HIF-1α protein were significantly greater in the immobilization and YC-1 groups than in the control group at 4 weeks. However, both expressions were significantly smaller in the YC-1 group than in the immobilization group at 4 weeks.
Conclusion |
From these results, the HIF-1α inhibitor may prevent immobilization-induced muscle fibrosis leading to muscle contracture. We surmised that the HIF-1α inhibition is a new therapeutic target for muscle contracture in immobilized skeletal muscle.
Le texte complet de cet article est disponible en PDF.Keywords : HIf-1α inhibitor, Muscle contracture, Fibrosis
Plan
Vol 61 - N° S
P. e412 - juillet 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.