Laundry detergents and detergent residue after rinsing directly disrupt tight junction barrier integrity in human bronchial epithelial cells - 04/05/19
Abstract |
Background |
Defects in the epithelial barrier have recently been associated with asthma and other allergies. The influence of laundry detergents on human bronchial epithelial cells (HBECs) and their barrier function remain unknown.
Objective |
We investigated the effects of laundry detergents on cytotoxicity, barrier function, the transcriptome, and the epigenome in HBECs.
Methods |
Air-liquid interface cultures of primary HBECs from healthy control subjects, patients with asthma, and patients with chronic obstructive pulmonary disease were exposed to laundry detergents and detergent residue after rinsing. Cytotoxicity and epithelial barrier function were evaluated. RNA sequencing, Assay for Transposase Accessible Chromatin with high-throughput sequencing, and DNA methylation arrays were used for checking the transcriptome and epigenome.
Results |
Laundry detergents and rinse residue showed dose-dependent toxic effects on HBECs, with irregular cell shape and leakage of lactate dehydrogenase after 24 hours of exposure. A disrupted epithelial barrier function was found with decreased transepithelial electrical resistance, increased paracellular flux, and stratified tight junction (TJ) immunostaining in HBECs exposed to laundry detergent at 1:25,000 dilutions or rinse residue at further 1:10 dilutions. RNA sequencing analysis showed that lipid metabolism, apoptosis progress, and epithelially derived alarmin-related gene expression were upregulated, whereas cell adhesion–related gene expression was downregulated by laundry detergent at 1:50,000 dilutions after 24 hours of exposure without substantially affecting chromatin accessibility and DNA methylation.
Conclusion |
Our data demonstrate that laundry detergents, even at a very high dilution, and rinse residue show significant cell-toxic and directly disruptive effects on the TJ barrier integrity of HBECs without affecting the epigenome and TJ gene expression.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Laundry detergent, rinse residue, barrier function, tight junction, bronchial epithelial cells, asthma, chronic obstructive pulmonary disease, transcriptome, epigenome
Abbreviations used : ALI, ATAC-seq, BP, COPD, DEG, DMP, FITC, GO, HBEC, LDH, RNA-seq, SDBS, TER, TJ, TSLP, UPLC, ZO-1
Plan
| Disclosure of potential conflict of interest: G. Tan, P. Wawrzyniak, P. Westermann, and A. Dreher are employed by the Swiss Institute of Allergy and Asthma Research. C. A. Akdis has consultant arrangements with Actellion, Aventis, Stallergenes, Allergopharma, and Circacia; is employed by the Swiss Institute of Allergy and Asthma Research; and has received grants from Novartis, PREDICTA: European Commission's Seventh Framework programme no. 260895, the Swiss National Science Foundation, MeDall: European Commission's Seventh Framework Programme no. 261357, and the Christine Kühne-Center for Allergy Research and Education. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 143 - N° 5
P. 1892-1903 - mai 2019 Retour au numéro
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