Syndecan-2 in colorectal cancer plays oncogenic role via epithelial-mesenchymal transition and MAPK pathway - 30/11/19
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 7 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | SDC2 is upregulated during CRC development. |
• | Knockdown of SDC2 holds back CRC cell proliferation in vitro. |
• | Knockdown of SDC2 suppresses CRC cell migration and invasion, and facilitates cell apoptosis in vitro. |
• | SDC2 promotes proliferation, migration and invasion, and activates EMT and MAPK signaling pathways in CRC cells. |
Abstract |
Purpose |
In this study, we aimed to elucidate the biological roles of Syndecan-2 (SDC2) in colorectal cancer (CRC), thereby further understanding its clinical role.
Methods |
The expression of SDC2 was assessed by qRT-PCR and Western blot analysis. To understand the potential biological role of SDC2, we also explored the correlation between its expression level and clinicopathologic parameters. By using MTT, plate colony formation assay, Transwell invasion assays, and flow cytometry in vitro, the biological impact of SDC2 on CRC cell proliferation, migration, invasion, and apoptosis. In addition, the related signaling pathways were investigated.
Results |
SDC2 expression was significantly upregulated in CRC tissues. The expression of SDC2 was highly associated with four parameters, i.e., stage (P < 0.01), vascular invasion (P = 0.0045), lymph node metastasis (P=0.0018), and distant metastasis (P = 0.0019). Knockdown of SDC2 significantly reduced proliferation, migration, and invasion of HCT116 and SW480 cells, and induced cell apoptosis. Moreover, SDC2 promoted epithelial-mesenchymal transition (EMT) in CRC cells, whereas the ratio of p-MEK/MEK and p-ERK/ERK markedly reduced after depleting SDC2.
Conclusion |
During CRC development, overexpression of SDC2 plays a carcinogenic role in CRC. Therapeutic solutions targeting SDC2 may provide potential insights into CRC prevention and treatment.
Le texte complet de cet article est disponible en PDF.Keywords : Syndecan-2, Colorectal cancer, Proliferation, Invasion, Epithelial-mesenchymal transition
Plan
Vol 121
Article 109630- janvier 2020 Retour au numéro
Article précédent
- RETRACTED: LNCRNA CDKN2B-AS1 regulates mesangial cell proliferation and extracellular matrix accumulation via miR-424-5p/HMGA2 axis
- Yang Li, Lin-lin Zheng, Deng-gao Huang, Hui Cao, Yuan-hui Gao, Zhong-cheng Fan
- Co-administration of omega-3 fatty acids and metformin showed more desirable effects than the single therapy on indices of bone mineralisation but not gluco-regulatory and antioxidant markers in diabetic rats
- Wale Johnson Adeyemi, Luqman Aribidesi Olayaki, Tahir Ahmad Abdussalam, Tolulope Olaoluwa Fabiyi, Temidayo Luqman Raji, Adenike Amatur-Rahman Adetunji
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?