Insight into SLC9A3 deficiency-mediated micturition dysfunction caused by electrolyte imbalance - 13/01/23
Abstract |
Background |
Solute carrier family nine isoform 3 (SLC9A3) is an Na+/H+ exchanger that regulates Ca2+ homeostasis. SLC9A3 is largely involved in the transepithelial absorption of Na+/H+ and frequently functions in pair with a Cl−/HCO3− exchanger.
Objective |
To investigate the impact and pathophysiological mechanisms of long-term SLC9A3 deficiency on lower urinary tract symptoms (LUTS) in a mouse model
Materials and methods |
Slc9a3 knockout and wild-type mice (average >6 months) were used. The effects of SLC9A3 depletion on bladder and urethral functions and effectiveness of voiding were assessed using a cystometrogram (CMG). Histology, blood electrolytes, and gene expression were also analyzed.
Results |
The SLC9A3-deficient mice had smaller gross bladders than the wild-type mice. The CMG analysis revealed normal peak micturition pressure, higher threshold pressure, short intercontraction interval, less voided volume, and poor compliance in the SLC9A3-deficient mice, similar to clinical LUTS. Histological analysis revealed loose detrusor muscle and loss of transformability of the urothelium in the SLC9A3-deficient mice. Masson's trichrome analysis revealed severe collagen deposition in the detrusor muscle. Immunofluorescence staining also demonstrated a significant decrease in cytokeratins 5 and 20. Gene and protein expression analyses confirmed that SLC9A3 does not act directly on bladder tissue. Homeostasis was correlated with bladder dysfunction in the SLC9A3-deficient mice.
Discussion |
Fibrosis and collagen deposition in the bladder of the SLC9A3-deficient mice is due to bladder inflammation because of decreased blood flow and deregulated systemic homeostasis. Long-term SLC9A3 depletion causes progressive bladder dysfunction, similar to human LUTS.
Conclusion |
Electrolyte imbalance causes SLC9A3 deficiency-mediated progressive micturition dysfunction.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Slc9a3−/− mice were confirmed to frequently urinate. |
• | Slc9a3−/− mice demonstrated collagen deposition and bladder fibrosis. |
• | Expression studies confirmed that SLC9A3 expression was high in renal tissues. |
• | Histological findings identified bladder barrier loss and urothelium damage. |
• | Electrolyte dysregulation causes homeostatic problems and LUTS. |
Keywords : Bladder dysfunction, Detrusor muscle atrophy, Electrolyte imbalance, SLC9A3
Plan
Vol 158
Article 114155- février 2023 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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