Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds - 28/03/23
Abstract |
Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity in diabetic patients. The standard treatment of DW care fails to address the prerequisites of treating DW owing to its multifactorial pathophysiology. Henceforth, developing a single treatment strategy to handle all the loopholes may effectively manage DW. The objective of the current study was to formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated in collagen/chitosan (COL-CS) composite scaffolds for the accelerated healing of DW. Upon investigation, the developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, and sustained drug release than the non-crosslinked scaffold. In vitro studies revealed that the HBD-2 COL-CS scaffold was biocompatible and accelerated cell migration and angiogenesis. The HBD-2 COL-CS scaffold showed significant antimicrobial activity in S. aureus, E. coli, and P. aeruginosa. The in vivo studies revealed that the HBD-2 COL-CS treated group accelerated healing compared to those in COL-CS and control groups. The ELISA results indicated a significant decrease in MMP-9, TNF-α, MPO, NAG, and NO with an increase in IL-10 in HBD-2 COL-CS treated group. The accelerated healing in HBD-2 COL-CS treated group might be due to the synergistic effects of PLGA (collagen synthesis and deposition and positive angiogenic effect), HBD-2 (anti-inflammatory, antibacterial, positive angiogenic effect, cell proliferation, and migration), COL (established wound healer and stabilizer) and CS (antibacterial, controlled drug release).
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
The present HBD-2 nanoparticles loaded acellular scaffold provides a strong base for future therapeutic approaches in skin regeneration strategies for treating diabetic wounds. The current formulation accelerated wound healing by suppressing inflammation and enhancing cell migration and angiogenesis.
The present HBD-2 nanoparticles loaded acellular scaffold provides a strong base for future therapeutic approaches in skin regeneration strategies for treating diabetic wounds. The current formulation accelerated wound healing by suppressing inflammation and enhancing cell migration and angiogenesis.ga1Le texte complet de cet article est disponible en PDF.
Keywords : Diabetic wound, Human beta defensin-2, Poly (lactic-co-glycolic acid), Nanoparticles, Scaffold
Plan
Vol 161
Article 114540- mai 2023 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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