Analytical concordance of Euroline® and ImmunoCAP® for peanut component-specific IgE - 02/11/26

Doi : 10.1016/j.jahd.2026.100078

Moïse Michel ^a,⁎ , Patricia Mathieu ^b, Oznur Sahin ^c, Mattéo Spatia ^c, Léa Sossah ^c, Rozerin Bilir ^c, Pascale Nicaise-Roland ^d, Pol-André Apoil ^e, Julien Goret ^f, Caroline Klingebiel ^b

On the behalf of the GTBA of the SFA

^a Desbrest Institute of Epidemiology and Public Health (IDESP), INSERM, Univ Montpellier, Laboratoire d’Immunologie, CHU Nîmes, Nîmes, France

^b Inovie Biofutur Laboratories, Conflans- Sainte- Honorine, France

^c Biology Laboratory Medical Montgrand 13006 Marseille, Marseille, France

^d Laboratoire d’immunologie, Hôpital Bichat-Claude Bernard (AP–HP), Paris, France

^e Institut Fédératif de Biologie, University Hospital Centre of Toulouse, Toulouse, France

^f Immunology Laboratory, CHU de Bordeaux, Bordeaux, France

^⁎ Corresponding author.

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Highlights

•	Determination of specific IgE to peanut molecular allergens is key for diagnosis and monitoring
•	Singleplex assays are widely used but sample consuming while line blot assay provides enables multiplex profiling
•	Line blot assays correlate with singleplex assays but shows important analytical bias
•	Line blot assays may provide molecular profiling with overall acceptable performance in peanut sIgE testing.

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Abstract

Background

Quantification of peanut allergen immunoglobulin E (IgE) is essential for the diagnosis and monitoring of peanut allergy. While singleplex assay is widely used for component-resolved diagnostic, line blot assay (LBA) may allow simultaneous detection of multiple allergen-specific IgEs. However, analytical concordance between these methods has not been extensively evaluated.

Methods

We conducted a retrospective multicentric study including 103 serum samples from children and adults with suspected peanut allergy. Specific IgE levels were measured using an ImmunoCAP platform (Ara h 1, 2, 3, 6, 8, and 9) and Euroline LBA (Ara h 1, 2, 3, 6, Bet v 1, and 9). Analytical agreement was assessed using Spearman correlation coefficients, Cohen’s kappa coefficients, and Bland–Altman analysis on log10-transformed data.

Results

Strong quantitative correlations were observed across all components (r = 0.765–0.889, p < 0.001). Qualitative agreement was fair to moderate (κ = 0.290–0.506). Bland–Altman analyses demonstrated substantial individual variability between methods.

Conclusion

Despite strong correlations, ImmunoCAP and Euroline showed limited qualitative agreement and wide limits of agreement, indicating that both methods are not directly interchangeable for quantitative peanut molecular IgE assessment. Nevertheless, Euroline may be useful for the broad characterization of component-specific IgE sensitization profiles, although its clinical utility remains to be established.

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Keywords : In vitro diagnosis, Line blot assay, Multiplex assay, Peanut allergy