Molecular genetic diversity and population structure in Lycium accessions using SSR markers - 10/12/10
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Abstract |
This study was conducted to assess the genetic diversity and population structure of 139 Lycium chinense accessions using 18 simple sequence repeat (SSR) markers. In total, 108 alleles were detected. The number of alleles per marker locus ranged from two to 17, with an average of six. The gene diversity and polymorphism information content value averaged 0.3792 and 0.3296, with ranges of 0.0793 to 0.8023 and 0.0775 to 0.7734, respectively. The average heterozygosity was 0.4394. The model-based structure analysis revealed the presence of three subpopulations, which was consistent with clustering based on genetic distance. An AMOVA analysis showed that the between-population component of genetic variance was less than 15.3%, in contrast to 84.7% for the within-population component. The overall FST value was 0.1178, indicating a moderate differentiation among groups. The results could be used for future L. chinense allele mining, association mapping, gene cloning, germplasm conservation, and designing effective breeding programs.
Le texte complet de cet article est disponible en PDF.Keywords : Lycium chinense Mill., Molecular diversity, Population structure, Simple sequence repeats (SSRs)
Abbreviations : SSR, PIC, RAPD, SCAR, AMOVA, UPGMA
Plan
Vol 333 - N° 11-12
P. 793-800 - novembre 2010 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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