Immune responses in adult female volunteers during the bed-rest model of spaceflight: Antibodies and cytokines - 15/08/11
Abstract |
Background |
It is unknown whether a prolonged period of bed rest will affect human immune responses, particularly in female subjects.
Objective |
We sought to measure immune responses in adult female subjects exposed to prolonged bed rest.
Methods |
Adult (25-40 years) female volunteers (n = 24) were maintained in a supine (6° tilt) head-down bed-rest (HDBR) position for 60 days: 8 with HDBR only, 8 with HDBR and regular muscular exercise, and 8 with HDBR and dietary protein supplementation. Subjects were immunized with bacteriophage X-174. Antibody production and plasma cytokine levels were determined.
Results |
The rate of primary antibody production of the HDBR plus exercise group increased faster (P = .01) and to a higher level versus that of the HDBR-only group (P = .03) and that of the HDBR plus diet group (trend P = .08). The rates of secondary antibody production between the 3 groups were similar, but the level of antibody in the HDBR plus exercise group remained higher versus that in the HDBR-only group (P = .03). Both the HDBR (P = .001) and HDBR plus diet (P = .02) groups had time-related progressive increases in TNF-⍺ receptor levels, but the HDBR plus exercise group remained at baseline. The HDBR plus exercise group experienced an acute increase in IL-1 receptor antagonist levels versus the HDBR (P = .02) and the HDBR plus diet (P = .02) groups, with similar increases in RANTES levels.
Conclusions |
The exercise countermeasure accelerated primary antibody production and increased antibody levels to bacteriophage X-174 and also opposed the potentially harmful effects of increased TNF-⍺ levels caused by prolonged bed rest, possibly by activating the anti-inflammatory cytokine IL-1 receptor antagonist and the chemotactic factor RANTES.
Le texte complet de cet article est disponible en PDF.Key words : Head-down tilt, bed rest, X-174 immunization, IgG class switching, spaceflight model, proinflammatory and anti-inflammatory cytokines, female study subjects
Abbreviations used : GEE, HDBR, IL-1RA, Kv, X-174, sTNFR1
Plan
Supported by the National Aeronautics and Space Administration through NASA Cooperative Agreement NCC 9-58 with the National Space Biomedical Research Institute. |
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Disclosure of potential conflict of interest: H. D. Ochs is on the advisory committee for Baxter and CSL-Behring and has received research support from CSL-Behring and the Modell Foundation. J. M. Reuben has received research support from the National Institutes of Health, the US Department of Defense, AstraZeneca, the Susan Komen Promise Grant, and the University of Texas, M.D. Anderson Cancer Center. B. Thompson has received research support from the National Institutes of Health. J. S. Butel has received research support from the National Institutes of Health as a principal investigator, coinvestigator, and project leader. G. Sonnenfeld has received research support from the National Aeronautics and Space Administration, the National Space Biomedical Research Institute, and Amino Up Chemical Company. H. Aviles has received support from the Amino Up Company. The rest of the authors have declared that they have no conflict of interest. |
Vol 123 - N° 4
P. 900-905 - avril 2009 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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