Absence of T-regulatory cell expression and function in atopic dermatitis skin - 15/08/11
Davos Platz and Zurich, Switzerland, Munich, Germany, and Utrecht, The Netherlands
Abstract |
Background |
The role of regulatory T cells has been widely reported in the suppression of T-cell activation. A dysfunction in CD4+CD25+ T-regulatory cell–specific transcription factor FoxP3 leads to immune dysregulation, polyendocrinopathy, enteropathy X-linked syndrome, often associated with atopic dermatitis. Increasing the number and activity of regulatory T cells in affected organs has been suggested as a remedy in various inflammatory diseases, including allergy.
Objective |
To determine the presence and function of regulatory T cells in atopic dermatitis.
Methods |
Immunohistochemistry of lesional atopic dermatitis skin and control skin conditions was used to demonstrate regulatory cells and cytokines in situ. The role of effector and regulatory T cells as well as their specific cytokines in apoptosis in human keratinocyte cultures and artificial skin equivalents was investigated.
Results |
Human T-regulatory type 1 cells, their suppressive cytokines, IL-10 and TGF-β, as well as receptors for these cytokines were significantly expressed, whereas CD4+CD25+FoxP3+ T-regulatory cells were not found in lesional and atopy patch test atopic dermatitis or psoriasis skin. Both subsets of regulatory T cells suppress the allergen-specific activation of TH1 and TH2 cells. In coculture and artificial skin equivalent experiments, subsets of T-regulatory cells neither induced keratinocyte death nor suppressed apoptosis induced by skin T cells, TH1 cells, IFN-γ, or TNF-⍺.
Conclusion |
A dysregulation of disease-causing effector T cells is observed in atopic dermatitis lesions, in association with an impaired CD4+CD25+FoxP3+ T-cell infiltration, despite the expression of type 1 regulatory cells in the dermis.
Le texte complet de cet article est disponible en PDF.Key words : Regulatory T cell, atopic dermatitis, apoptosis, suppression, regulation, skin, human, inflammation
Abbreviations used : AD, APT, FasL, HDM, NAD, Tr1, Treg
Plan
Authors’ laboratories supported by Swiss National Science Foundation grants No. 31-105865 and 32-100266 and the Global Allergy and Asthma European Network (GA2LEN). |
Vol 117 - N° 1
P. 176-183 - janvier 2006 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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