Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse - 19/08/11

Doi : 10.1016/j.ajog.2010.02.053

Peter Takacs, MD, PhD ^a,^b, Marc Gualtieri, MD ^a, Mehdi Nassiri, MD ^d, Keith Candiotti, MD ^a,^b, Alessia Fornoni, MD, PhD ^c, Carlos A. Medina, MD ^a
^a Department of Obstetrics and Gynecology, Miller School of Medicine, University of Miami, Miami, FL
^b Department of Anesthesiology, Miller School of Medicine, University of Miami, Miami, FL
^c Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL
^d Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN

Résumé

Objective

To compare smooth muscle regulatory protein expression in the uterosacral ligament (USL) of women with and without uterine prolapse.

Study Design

USLs ligament were sampled in women with (n = 9) or without (n = 9) uterine prolapse. Caldesmon, smooth muscle actin (SMA), myosin heavy chain, and zinc finger protein messenger RNA expression was assessed by quantitative real-time polymerase chain reaction. Immunohistochemistry and digital image analysis were used to determine protein expression.

Results

Caldesmon messenger RNA expression and the ratio of caldesmon-SMA messenger RNA expression was significantly increased in the USL from women with uterine prolapse compared with women without prolapse (caldesmon mean ± standard deviation messenger RNA, 0.81 ± 0.46 vs 0.39 ± 0.16; P = .01 and caldesmon-SMA messenger RNA ratio, mean ± standard deviation, 0.11 ± 0.04 vs 0.07 ± 0.02; P = .01). In addition, the ratio of caldesmon-SMA staining was significantly increased in women with uterine prolapse compared with women without prolapse (mean ± standard deviation, 0.44 ± 0.28 vs 0.28 ± 0.16; P = .03).

Conclusion

Uterine prolapse is associated with an increased ratio of caldesmon-SMA actin expression.

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Key words : caldesmon, prolapse, smooth muscle cell, uterosacral ligament

Plan

Materials and Methods

Tissue preparation

Immunohistochemistry

Image analysis: determination of nonvascular smooth muscle fractional area

RNA extraction

Real-time polymerase chain reaction

Statistical methods

Results

Comment

	Reprints not available from the authors.
	Cite this article as: Takacs P, Gualtieri M, Nassiri M, et al. Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse. Am J Obstet Gynecol 2010;202:620.e1-5.

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Vol 202 - N° 6

P. 620.e1-620.e5 - juin 2010 Retour au numéro

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Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse - 19/08/11

Résumé

Objective

Study Design

Results

Conclusion

Plan

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