Altered gene expression patterns in intrauterine growth restriction: Potential role of hypoxia - 22/08/11
Résumé |
Objective |
Placental insufficiency is a primary cause of intrauterine growth restriction (IUGR). In our study, microarray technology was used to identify genes, which may impair placentation resulting in IUGR.
Study design |
The RNA was isolated from both IUGR term placentas and normal term placentas. Microarray experiments were used to identify differentially expressed genes between the 2 cohorts. Real-time quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry were used in follow-up experiments.
Results |
Microarray experiments identified increased expression of certain genes including leptin, soluble vascular endothelial growth factor receptor, human chorionic gonadotropin, follistatin-like 3, and hypoxia-inducible factor 2⍺ in the IUGR. Real-time quantitative polymerase chain reaction confirmed these results.
Conclusion |
The upregulation of soluble vascular endothelial growth factor receptor and hypoxia-inducible factor 2⍺ at this period in pregnancy indicate that placental angiogenesis is altered in IUGR and that hypoxia is a major contributor to maldevelopment of the placental vasculature.
Le texte complet de cet article est disponible en PDF.Key words : angiogenesis, hypoxia, intrauterine growth restriction, placentation
Plan
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| Cite this article as: McCarthy C, Cotter FE, McElwaine S, et al. Altered gene expression patterns in intrauterine growth restriction: Potential role of hypoxia. Am J Obstet Gynecol 2007:196:70.e1-70.e6. |
Vol 196 - N° 1
P. null - janvier 2007 Retour au numéro
Article précédent
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