Increased sensitivity of asthmatic airway smooth muscle cells to prostaglandin E2 might be mediated by increased numbers of E-prostanoid receptors - 24/08/11
Sydney, Australia
Abstract |
Background |
An increase in airway smooth muscle (ASM) cell proliferation leads to an increase in the bulk of the ASM, one of the characteristic features of asthma. We have previously shown that ASM cells from asthmatic individuals proliferate more than those from nonasthmatic subjects. This increased growth might be due to compromised inhibitory mechanisms within the ASM of asthmatic subjects.
Objective |
The purpose of this study was to determine whether the proliferative control exerted by prostaglandin E2 (PGE2) was altered in the asthmatic ASM cells.
Methods |
We used tritated thymidine uptake to measure cell proliferation and cell-surface ELISAs to detect the presence of cell-surface receptors on ASM cells isolated from asthmatic and nonasthmatic individuals.
Results |
The asthmatic ASM cells were significantly more sensitive to proliferation inhibition by PGE2 than the nonasthmatic cells (P<.02). The PGE2 (E-prostanoid [EP]) receptors EP2 and EP3 were detected on asthmatic and nonasthmatic smooth muscle cells in culture. There were significantly more receptors on the asthmatic cells. The asthmatic cells also had increased sensitivity to proliferation inhibition by EP2-specific agonists but not by EP3-specific agonists.
Conclusion |
The increased growth observed in asthmatic ASM cells is not the result of impaired responsiveness to PGE2. In contrast, these cells have increased sensitivity. This increased sensitivity might be mediated by the increased numbers of EP2 receptors on the surface.
Le texte complet de cet article est disponible en PDF.Keywords : Asthma, prostaglandin E2, proliferation, EP receptors, airway smooth muscle
Abbreviations : ASM, C/EBP, DMEM, EP receptor, Ki, PGE2, PGI2
Plan
Supplementary data associated with this article can be found at doi:10.1016/j.jaci.2004.02.029. Supported by the National Health and Medical Research Council, Australia. J. K. Burgess is supported by an NH&MRC Peter Doherty Fellowship (no. 165722). |
Vol 113 - N° 5
P. 876-881 - mai 2004 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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