Increased adhesion to vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 of eosinophils from patients with asthma - 12/09/11
Abstract |
Adhesion of peripheral blood eosinophil and neutrophil granulocytes to the endothelial cell adherence receptors E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 has been measured. The study included patients with allergic rhinitis, patients with mild allergic and nonallergic asthma, and healthy individuals; 10 persons were in each group. In addition, assay of eosinophil and neutrophil cell surface expression of the receptor complex CD11b/CD18 was performed. Increased eosinophil adhesion to vascular cell adhesion molecule-1 (p < 0.05) and intercellular adhesion molecule-1 (p < 0.05) was demonstrated in the patients with a more labile asthma, that is, a peak expiratory flow rate variability of more than 10%, suggesting a relationship to the degree of ongoing inflammation in the airways of the patients. The increased eosinophil adhesion was most probably due to a functional upregulation of the CD11b/CD18 and very late activation antigen-4 receptors, because the number of receptors measured as cell surface expression was unaltered. The increased eosinophil adhesion in the patients with high peak expiratory flow rate variability appeared independent of atopy. The increased adhesion was not entirely specific to the eosinophils, because neutrophils from patients with a peak expiratory flow rate variability of more than 10% also demonstrated increased adhesion to intercellular adhesion molecule-1 (p < 0.05) when compared with neutrophils from the patients with low peak expiratory flow rate variability. In conclusion, the demonstrated priming of eosinophil adhesion to vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 might be one contributing mechanism behind the selective accumulation of eosinophils in the lung tissue of patients with asthma. (J ALLERGY CLIN IMMUNOL 1995;96:941-50.)
Le texte complet de cet article est disponible en PDF.Keywords : Eosinophil, neutrophil, adhesion, E-selectin, VCAM-1, ICAM-1, CD11b/CD18, VLA-4, asthma
Abbreviations : BHK, BHR, ECP, FEV1, ICAM, MPO, PD20, PEF, VCAM, VLA
Plan
From the aDepartment of Clinical Chemistry, bthe Department of Lung Medicine, and cAsthma Research Centre, University Hospital, Uppsala; and dthe Department of Clinical Physiology, University Hospital, Linköping. |
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Supported by grants from the Medical Faculty of the University of Uppsala, Bror Hjerpstedts Stiftelse, the National Association against Asthma and Allergy, the Swedish Heart-Lung Foundation, and the Swedish Medical Research Council. |
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Reprint requests: Lena Håkansson, PhD, Laboratory for Inflammation Research, Department of Clinical Chemistry, University Hospital, S-751 85 Uppsala, Sweden. |
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1/1/64684 |
Vol 96 - N° 6
P. 941-950 - décembre 1995 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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