A detective monoclonal antibody recognized conserved epitope of hemagglutinin from H5N1 influenza virus - 24/09/11

Doi : 10.1016/j.crci.2011.04.001

Xiaoxiao Zhuang ^b,^{^{1
These authors contributed equally to this work.}}, Yizhuo Sun ^b,^{^{1
These authors contributed equally to this work.}}, Zhiyang Ling ^a,^{^{1
These authors contributed equally to this work.}}, Qingxi Dong ^b, Jingyan Zhu ^c, Linna Tang ^c, Yanyan Zhou ^c, Yongyong Ji ^b, Lin Tian ^b, Guomei Lin ^b, Lingchen Yan ^b, Chaofeng Huang ^b, Ze Chen ^d, Chao Bian ^b,^⁎ , Bing Sun ^a,^b,^⁎
^a Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, China
^b Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
^c Shanghai Immune Biotech Co. Ltd., Shanghai 200233, China
^d Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

Corresponding author.Co-corresponding author.

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

pages	5
Iconographies	6
Vidéos	0
Autres	0

Abstract

Monoclonal antibodies are needed for the development of effective, accurate and sensitive immunoassays against the H5N1 avian influenza. Here we report the generation of monoclonal antibodies targeting Escherichia coli expressed hemagglutinin of HA1 domain from H5N1 influenza virus. The monoclonal antibodies can be used to establish a sandwich ELISA assay that is able to detect HA from H5 subtype virus lysate but not from H9 subtype. A subtype-conserved new epitope was also identified which is located between the amino acid residues N80 to A99. This work would be helpful for the development of a diagnosis kit for the H5N1 avian influenza.

Le texte complet de cet article est disponible en PDF.

Keywords : Immunoassays, Immunology, Proteins, Antigens, Antibodies