Cell proliferation in ventromedial hypothalamic lesioned rats inhibits acute gastric mucosal lesions - 07/08/12
, Akira Senoo b, Junko Sakurai c, Masaru Sonoda d, Masao Kanazawa e, Yoko Suzuki a, Yoko Kobayashi a, Tosei Takahashi a, Ryota Haba a, Katsumi Arai a, Hiroyuki Shimizu a, Kahoru Sasaki b, Masako Kako b, Kaori Hayashi b, Yuichi Suzuki f, Shuji Inoue a
Corresponding author at: Department of Nutrition, Faculty of Health Care, Kiryu University, 606-7 Azami, Kasakake-cho, Midori-shi, Gunma 379-2392, Japan. Tel.: +81 277 48 9121; fax: +81 277 76 9454.Abstract |
Aim |
The role of mucosal layer thickness on prevention of acute gastric mucosal lesions (AGMLs) was examined in ventromedial hypothalamic (VMH)-lesioned rats.
Materials and methods |
The incidence of AGMLs after 48-h fasting and 60% ethanol injection into the stomach after 24-h fasting, aggressive factors (gastric acid and serum gastrin) and defensive factors [hexosamine, gastric mucosal blood flow (GMBF), serum thiobarbituric acid reacting substances (TBARS), and thickness of the gastric mucosal layer] were evaluated in VMH-lesioned rats. The effects of cell proliferation on the gastric mucosal layer of these rats were evaluated by H–E staining and immunostaining with proliferating cell nuclear antigen (PCNA).
Results |
After 48-h fasting, no AGMLs were observed in VMH-lesioned and sham VMH-lesioned rats (controls). With 60% ethanol administration after 24-h fasting, the numbers of AGMLs were similar in the two groups, but the ulcer index, a marker of ulcer formation, was lower in VMH-lesioned rats compared to that in sham VMH-lesioned rats. VMH-lesioned rats showed increased gastric acid secretion and serum gastrin compared to sham VMH-lesioned rats, indicating an increase in aggressive factors in VMH-lesioned rats. The two groups had similar levels of gastric mucosal hexosamine, GMBF, and gastric mucosal TBARS, but VMH-lesioned rats had an increased thickness of the mucosal cell layer, indicating an increase in defensive factors in these rats. Histologically, VMH-lesioned rats had an increased total mucosal cell layer, especially for the surface epithelial cell layer, and an increased PCNA-labeling index, a marker of cell proliferation, especially in the proliferative zones of gastric mucosa, indicating increased cell proliferation in the proliferative zone of the gastric mucosa.
Conclusion |
VMH-lesioned rats are resistant to AGML formation due to increased cell proliferation in gastric mucosa through elevating the levels of defensive factors over those of aggressive factors.
Le texte complet de cet article est disponible en PDF.Keywords : AGML, Ventromedial hypothalamic lesions, Rat, Ulcer index, Aggressive factor, Defensive factor, Proliferating cell nuclear antigen (PCNA)-labeling index, Cell proliferation
Plan
Vol 6 - N° 3
P. e233-e240 - juillet 2012 Retour au numéro
Article précédent
- Masked function of amino acid sensors on pancreatic hormone secretion in ventromedial hypothalamic (VMH) lesioned rats with marked hyperinsulinemia
- Noriko Ishizuka, Katsuaki Tanaka, Yoko Suzuki, Yuri Kintaka, Ikiko Kinoshita, Takeo Hashiguchi, Hiroyuki Shimizu, Akira Senoo, Nobuo Imazeki, Yoko Kobayashi, Katsumi Arai, Ryota Haba, Tosei Takahashi, Kahoru Sasaki, Masako Kako, Kaori Hayashi, Toshimasa Osaka, Yuichi Suzuki, Shuji Inoue
- Ventromedial hypothalamic lesions enhance small intestinal cell proliferation in mice
- Noriko Ishizuka, Akira Senoo, Kaori Hayashi, Kahoru Sasaki, Masako Kako, Yoko Suzuki, Nobuo Imazeki, Hiroyuki Shimizu, Yoko Kobayashi, Ryota Haba, Tosei Takahashi, Katsumi Arai, Toshimasa Osaka, Yuri Kintaka, Yuichi Suzuki, Shuji Inoue
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