Fabricated autologous epidermal cell sheets for the prevention of esophageal stricture after circumferential ESD in a porcine model - 17/09/12
Reprint requests: Teruo Okano, PhD, Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-kku, Tokyo 162-8666 JapanRésumé |
Background |
Endoscopic submucosal dissection (ESD) is an accepted treatment for early esophageal carcinoma. However, resection of a large mucosal area, as with circumferential ESD, induces severe stricture formation.
Objective |
To evaluate the efficacy of cultured autologous epidermal cell sheets to prevent severe esophageal constriction after circumferential ESD.
Design |
Animal study.
Setting |
University institute.
Intervention |
Eight pigs underwent circumferential esophageal ESD while under general anesthesia. In 4 pigs, fabricated autologous epidermal cell sheets were endoscopically transplanted to the central ESD sites immediately after the ESD. The other 4 pigs underwent circumferential ESD only. Necropsy and histological assessment were performed at 1 and 2 weeks post-ESD.
Main Outcome Measurements |
Weight gain, degree of mucosal constriction, and histological assessments.
Results |
All pigs in the control group showed severe esophageal constriction after 2 weeks. The control and transplanted groups had weight gains of −10.3% and 0.3% (P = .03), respectively, and the mean degrees of constriction were 88% and 56% (P < .01), respectively. Early re-epithelialization and mild fibrosis in the muscularis were observed in the transplanted group.
Limitations |
Animal study, small sample size.
Conclusions |
Fabricated autologous skin epidermal cell sheets would be useful in preventing severe esophageal constriction after circumferential ESD.
Le texte complet de cet article est disponible en PDF.Abbreviations : ESD, SD
Plan
| DISCLOSURE: The following authors disclosed financial relationships relevant to this publication: Dr. Yamato, consultant to CellSeed; Dr. Okano, investor in CellSeed. The other authors disclosed no financial relationships relevant to this publication. |
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| This study was partially supported by the Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology Cell Sheet Tissue Engineering Center and the Global COE program, the Multidisciplinary Education and Research Center for Regenerative Medicine of the Ministry of Education, Culture, Sports, Science and Technology, Japan. |
Vol 76 - N° 4
P. 873-881 - octobre 2012 Retour au numéro
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