Association of bactericidal activity of genital tract secretions with Escherichia coli colonization in pregnancy - 27/09/12
Résumé |
Objective |
Genital tract secretions exhibit bactericidal activity against Escherichia coli. We hypothesized that this defense may be modulated during pregnancy.
Study Design |
Secretions were collected by vaginal swab from 70 pregnant women (35-37 weeks' gestation) and 35 nonpregnant controls. We mixed E coli with swab eluants or control buffer and colonies enumerated to measure bactericidal activity. Cytokines, chemokines, and antimicrobial peptides were quantified by multiplex or enzyme-linked immunosorbent assay.
Results |
Pregnant women had significantly greater bactericidal activity, higher concentrations of proinflammatory cytokines, and lower levels of beta defensins compared to controls. Seven (10%) pregnant and 8 (23%) nonpregnant women were vaginally colonized with E coli; colonization was inversely associated with bactericidal activity.
Conclusion |
The soluble mucosal immune environment is altered in pregnancy. We speculate that the observed changes may protect against colonization and ascending infection and could provide a biomarker to identify pregnant women at risk for infectious complications including preterm birth.
Le texte complet de cet article est disponible en PDF.Key words : cytokines/chemokines, defensins, Escherichia coli, pregnancy, soluble mucosal immunity in genital tract
Plan
| This research was supported by research grant numbers UL1 RR025750, AI076980, AI079763, and UL1 RR025750 (all B.C.H.) and KL2 RR025749 (J.P.G.). |
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| The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the National Institutes of Health. |
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| The authors report no conflict of interest. |
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| Cite this article as: Ghartey JP, Carpenter C, Gialanella P, et al. Association of bactericidal activity of genital tract secretions with Escherichia coli colonization in pregnancy. Am J Obstet Gynecol 2012;207:297.e1-8. |
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| Reprints not available from the authors. |
Vol 207 - N° 4
P. 297.e1-297.e8 - octobre 2012 Retour au numéro
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