Amniotic fluid induces platelet-neutrophil aggregation and neutrophil activation - 25/03/13
Reprints: Chi-Yuan Li, Department of Anesthesiology, China Medical University Hospital, 2, Yuh-Der Rd., Taichung, Taiwan, 40447Résumé |
Objective |
Amniotic fluid embolism syndrome is a fatal disease in pregnant women. The exact role of platelets and neutrophils in amniotic fluid embolism syndrome is not clear. We examined whether amniotic fluid could affect platelet-neutrophil aggregation and activation and the possible mechanisms.
Study Design |
Blood samples from the pregnant women were pretreated ex vivo with their own amniotic fluid. Flow cytometry was used to measure platelet-neutrophil aggregation and activation. Neutrophil-mediated activity of p38 mitogen-activated protein kinase and extracellular signal-regulated protein kinases 1 and 2 was analyzed by Western blotting.
Results |
Amniotic fluid significantly induced platelet-neutrophil aggregation, neutrophil CD11b expression, and reactive oxygen species production. Amniotic fluid induced minimal platelet P-selectin expression. The increase of intracellular calcium level of neutrophils and the activity of p38 mitogen–activated protein kinase were enhanced by amniotic fluid stimulation.
Conclusion |
Amniotic fluid was able to induce neutrophil activation and platelet-neutrophil aggregation with minimal effect on platelet activation. These findings may provide a new insight in the understanding of the pathophysiologic condition of amniotic fluid embolism syndrome.
Le texte complet de cet article est disponible en PDF.Key words : amniotic fluid embolism syndrome (AFES), neutrophil activation, p38 mitogen-activated protein kinase (MAPK), platelet-neutrophil aggregation
Plan
| Supported in part by grants from the Department of Defense (DOD98-09-06), the China Medical University Hospital (DMR-100-101), and the Taiwan Department of Health Clinical Trial and Research Center of Excellence (DOH100-TD-B-111-004), Taiwan, Republic of China. |
|
| The authors report no conflict of interest. |
|
| Cite this article as: Chen K-B, Chang S-S, Tseng Y-L, et al. Amniotic fluid induces platelet-neutrophil aggregation and neutrophil activation. Am J Obstet Gynecol 2013;208:318.e1-7. |
Vol 208 - N° 4
P. 318.e1-318.e7 - avril 2013 Retour au numéro
Article précédent
- Developmental programming for allergy: a secondary analysis of the Mothers, Omega-3, and Mental Health Study
- Vivian C. Romero, Emily C. Somers, Valerie Stolberg, Chelsea Clinton, Stephen Chensue, Zora Djuric, Deborah R. Berman, Marjorie C. Treadwell, Anjel M. Vahratian, Ellen Mozurkewich
- Pregnancy-induced changes in immune protection of the genital tract: defining normal
- Brenna L. Anderson, Hector Mendez-Figueroa, Joshua D. Dahlke, Christina Raker, Sharon L. Hillier, Susan Cu-Uvin
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?