Inhibition of preadipocyte differentiation and lipid accumulation by 7-O-galloyl-d-sedoheptulose treatment in 3T3-L1 adipocytes - 07/12/13

Doi : 10.1016/j.bionut.2013.03.012

Chan Hum Park ^a,^{^{1
These two authors contributed equally to the study.}}, Dong Young Rhyu ^b,^{^{1
These two authors contributed equally to the study.}}, Bhesh Raj Sharma ^b, Takako Yokozawa ^a,^c,^⁎
^a Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
^b College of Natural Sciences and Institute of Korean Traditional Medicine Industry, Mokpo National University, 61, Dorim-ri, Muan-gun, Jeonnam 534-729, South Korea
^c Organization for Promotion of Regional Collaboration, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan

^⁎Corresponding author. Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. Tel.: +81 76 445 6667; fax: +81 76 445 6549.

Abstract

In this study, we investigated the effect of 7-O-galloyl-d-sedoheptulose (GS) on adipocyte differentiation in 3T3-L1 cells. GS significantly suppressed 3T3-L1 differentiation. The activation of adenosine monophosphate-activated protein kinase (AMPK) was demonstrated by measuring phosphorylation. In addition, GS down-regulated the adipogenesis-related protein expression of the sterol regulatory element-binding protein 1c. GS significantly reduced the protein expression of the peroxisome proliferator-activated receptor γ and of the CCAAT/enhancer-binding protein α, two key transcription factors in adipogenesis. These findings indicate that GS exerts anti-adipogenic activity via AMPK activation and may act to prevent obesity.

Le texte complet de cet article est disponible en PDF.

Keywords : 7-O-Galloyl-d-sedoheptulose, 3T3-L1 cell, Adipogenesis, AMPK, SREBP1c, PPARγ, C/EBPα

Plan

Introduction

Materials and methods

Chemicals and materials

Preparation of Cornus officinalis fractions and purification of 7-O-galloyl-d-sedoheptulose

Cell culture and differentiation

MTT assay

Oil red O staining

Western blot analyses

Statistical analysis

Results

Effects of 7-O-galloyl-d-sedoheptulose on adipocyte differentiation

Effects of 7-O-galloyl-d-sedoheptulose on adenosine monophosphate-activated protein kinase phosphorylation

Effects of 7-O-galloyl-d-sedoheptulose on peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α expressions

Effects of 7-O-galloyl-d-sedoheptulose on sterol regulatory element-binding protein-1c expression

Discussion

Disclosure of interest

Export

Vol 3 - N° 4

P. 319-324 - octobre 2013 Retour au numéro

Article précédent

Protective role of coenzyme Q₁₀, riboflavin, niacin, selenium (CoRNS) and Emblica officinalis on cardiac abnormalities in experimental atherosclerosis
Umapathi Indumathi, Karvannan Kanchana, Panchanatham Sachdanandam

| Article suivant

Design, synthesis and biological evaluation of some isatin-linked chalcones as novel anti-breast cancer agents: A molecular hybridization approach
Chandrabose Karthikeyan, Viswas Raja Solomon, Hoyun Lee, Piyush Trivedi

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

Inhibition of preadipocyte differentiation and lipid accumulation by 7-O-galloyl-d-sedoheptulose treatment in 3T3-L1 adipocytes - 07/12/13

Abstract

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL