Background and Aims: Electronic image processing has made it possible to display objects in three dimensions. Three-dimensional (3 D) reconstruction of images has been applied to some imaging modalities (US, EUS, CT, MRI) and potential advantages over conventional techniques have been reported.With flexible endscopy gastrointestinal structures can be viewed from multiple angles. However, standard endoscopy allows only the display of images in two dimensions (2D). Using a new software, we assessed the feasibility of 3 D reconstruction of endoscopic images. Methods: Endoscopy was performed with a standard endoscope and video camera in six patients with rectal carcinoma. rectal villous adenoma, pneumatosis cystoides intestinalis or esophageal carcinoma. Image acquisition was performed during slow withdrawal of the endoscope. The video signal was transmitted to a PC work station, and 3 D reconstruction of endoscopic images was performed using a 3D software (Freescan Easy 3 D. EchoTech 3 D Imaging Systems GmbH, Halbergmoos. Germany) Results 3 D reconstruction was possible in all patients. but only in straight areas in the rectum and esophagus In these organs. images could be displayed in many planes allowing a more global view of the lesions. Conclusions: 3 D reconstruction of endoscopic images is feasible and high resolution images can be obtained with this method. Further studies should assess whether 3D endoscopy provides any additional useful information compared to standard endoscopy.