Nitric Oxide Modulatory Mechanism in the Protective Effect of Retigabine Against Spinal Nerve Ligation Induced Neuropathic Pain - 09/06/15
Résumé |
Introduction |
Decreasing the hyper excitability of neurons through opening of voltage-gated potassium (Kv7, also termed as KCNQ) channels has been suggested as one of the protective mechanisms in the effective management of neuropathic pain. Reactive oxygen/nitrogen species and inflammatory pathways are well implicated in the pathophysiology of neuropathic pain. Further, M current generated by opening of KCNQ channels has been modulated by reactive oxygen/nitrogen species.
Aim & Objectives |
The present study has been designed to elucidate the nitric oxide modulatory mechanism in the protective effect of retigabine against spinal nerve ligation induced neuropathic pain in rats.
Methods |
Ligation of L5 and L6 spinal nerves resulted alterations in various behavioral (as evident from marked increase in thermal and mechanical hyperalgesia and allodynia), biochemical (raised lipid peroxidation, nitrite, & depletion of GSH, SOD, catalase) and inflammatory parameters (raised TNF-alpha) as compared to naive treatment.
Results |
Administration of retigabine (10 mg/kg) for 28 days attenuated these behavioral, biochemical and inflammatory cascades as compared to control. Further, L-arginine (100 mg/kg) pretreatment with retigabine (5 mg/kg) significantly reversed the protective effect of retigabine in spinal nerve ligated rats. However, L-NAME (10 mg/kg) pretreatment with retigabine (5 mg/kg) significantly potentiated their protective effects which were significant as compared to their effect per se respectively.
Conclusions |
Present study highlights the involvement of nitric oxide mechanism in the protective effect of retigabine against L5/L6 spinal nerve ligation induced behavioral and biochemical alterations in rats.
Le texte complet de cet article est disponible en PDF.Vol 30 - N° S1
P. 328 - mars 2015 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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