Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency - 10/09/15

Doi : 10.1016/j.ajo.2015.06.026

Eric H. Chan ^a,^b, Luxia Chen ^a, Jian Yu Rao ^c, Fei Yu ^d, Sophie X. Deng ^a,^⁎
^a Cornea Division, Stein Eye Institute, University of California, Los Angeles, Los Angeles, California
^b David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California
^c Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, California
^d Department of Biostatistics, University of California, Los Angeles, Los Angeles, California

^∗Inquiries to Sophie X. Deng, Cornea Division, Stein Eye Institute, University of California, Los Angeles, 100 Stein Plaza, Los Angeles, CA 90095

Abstract

Purpose

To investigate changes in limbal basal epithelial cell density in eyes with limbal stem cell deficiency (LSCD) using in vivo confocal laser scanning microscopy.

Design

Retrospective observational comparative study.

Methods

A total of 43 eyes of 30 patients diagnosed with LSCD were included in the study. Ten eyes from normal subjects were included as control. Confocal imaging of the central cornea, and the superior, nasal, inferior and temporal limbus were collected using the Heidelberg Retina Tomograph III Rostock Corneal Module. Basal cell density in all locations was measured by 2 independent observers.

Results

The mean basal cell density of the normal group was 9264 ± 598 cells/mm² in the cornea and 7120 ± 362 cells/mm² in the limbus. In the LSCD group, the mean basal cell density in the cornea decreased 31.0% (6389 ± 1820 cells/mm², P < .001) and in the limbus decreased 23.6% (5440 ± 1123 cells/mm², P < .001) compared to that in the control. There was a trend of basal cell density decline in more advanced stages of LSCD. The basal cell density declined in the unaffected regions at a similar degree as that in the affected region in sectoral LSCD (P > .05). The basal cell diameter increased by 24.6% in the cornea (14.7 μm) and by 15.7% in the limbus (15.5 μm) compared to the control.

Conclusions

Basal cell density in both central cornea and limbus decreases in LSCD. Limbal stem cells (LSCs) are affected globally and basal cell density could be used as a parameter to measure LSC function at the early stages of the disease process.

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Plan

Methods

Confocal Microscopy Analysis

Statistical Analysis

Results

Discussion

	Supplemental Material available at AJO.com.
	Luxia Chen is currently affiliated with Tianjin Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, People's Republic of China.

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Vol 160 - N° 4

P. 678 - octobre 2015 Retour au numéro

Article précédent

Epithelial Thinning in Limbal Stem Cell Deficiency
Eric H. Chan, Luxia Chen, Fei Yu, Sophie X. Deng

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Limbal Basal Cell Density Decreases in Limbal Stem Cell Deficiency - 10/09/15

Abstract

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Results

Conclusions

Plan

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