Lobetyol activate MAPK pathways associated with G₁/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo - 02/06/16

Doi : 10.1016/j.biopha.2016.03.046

Jie Shen ^a,^{^{1
These authors contributes equally to this work.},}^{^{2
Permanent address: Department of Pharmacy, HuaDong Hospital, FuDan University, Shanghai 200040, PR China.}}, XinGang Lu ^b,^{^{1
These authors contributes equally to this work.}}, WangChun Du ^c, Jun Zhou ^d, HongFu Qiu ^b, JingXian Chen ^e, XiaoHeng Shen ^e, MingKang Zhong ^a,^⁎,^{^{1
These authors contributes equally to this work.}}
^a Clinical Pharmacy Laboratory, HuaShan Hospital, FuDan University, Shanghai 200040, PR China
^b Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University, Shanghai 200040, PR China
^c Department of Clinical Medicine, Shanghai University of Medicine & Health Sciences, Shanghai 200040, PR China
^d Department of Gastric, HuaDong Hospital, FuDan University, 200040, PR China
^e Department of Traditional Chinese Medicine, RuiJin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, PR China

^⿿Corresponding author at: Department of Pharmacy, HuaShan Hospital, Fudan University, 12 Wu Lu Mu Qi Rd, Shanghai 200040, China.

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Highlights

⿢	Lobetyol is a natural agent isolated from herb plant Lobelia chinensis.
⿢	Lobetyol induce apoptosis and cell cycle arrest on MKN45 cells.
⿢	The progression was mediated by the activation of MAPK family pathway.

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Abstract

Aim

The agent lobetyol, which is isolated from Lobelia chinensis, was previously shown to be cytotoxicity againts several cancer cell lines in published report. Today, we perform a study in vitro and in vivo to analyze its anti-carcinoma effect in MKN45 cells and to explore the molecular mechanism.

Main methods

The growth inhibition of lobetyol on MKN45 cells was analyzed with MTT and flow cytometry. Hoechst 33342 staining and TUNEL cover glass staining were used to provide the visual evidence of apoptosis. Western blotting assay was performed to study the activation or blocking of related signaling pathways.

Key findings

Lobetyol induce apoptosis and cell cycle arrest in a time- and dose-dependent manner in MKN45 cells in our study. This process is mediated by the MAPK signaling pathways.

This study confirmed the cytotoxicity of lobetyol in MKN45 cells and provided an insight into the molecular mechanism, which demonstrates the potential of lobetyol as an anti-tumor agent.

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Abbreviation : p

Keywords : MKN45, Apoptosis, Cell cycle arrest, Lobetyol, MAPK

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Vol 81

P. 120-127 - juillet 2016 Retour au numéro

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Lobetyol activate MAPK pathways associated with G₁/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo - 02/06/16

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