Genome-based study of a spatio-temporal cluster of invasive meningococcal disease due to Neisseria meningitidis serogroup C, clonal complex 11 - 10/07/16

Doi : 10.1016/j.jinf.2016.05.003

P. Stefanelli ^a,^⁎ , C. Fazio ^a,^{^{h
These authors contributed equally to this article.}}, A. Neri ^a,^{^{h
These authors contributed equally to this article.}}, A. Ciammaruconi ^b, E. Balocchini ^c, A. Anselmo ^b, C. Azzari ^d, G.M. Rossolini ^e,^f,^g, P. Vacca ^a, A. Fortunato ^b, A. Palozzi ^b, S. Fillo ^b, F. Lista ^b, M. Moriondo ^d, F. Nieddu ^d, G. Rezza ^a
^a Department of Infectious, Parasitic & Immuno-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
^b Molecular Biology Section, Army Medical and Veterinary Research Center, Rome, Italy
^c Living Environment, Food and Veterinary Prevention and Safety Office, Tuscany Region, Florence, Italy
^d Laboratory of Immunology and Infectious Diseases, Anna Meyer Children's University Hospital, University of Florence, Florence, Italy
^e Department of Medical Biotechnologies, University of Siena, Italy
^f Department of Experimental and Clinical Medicine, University of Florence, Italy
^g Microbiology and Virology Unit, Florence Careggi University Hospital, Italy

^∗Corresponding author. Department of Infectious, Parasitic & Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Tel.: +39 06 49902126; fax: +39 06 49387112.Department of Infectious, Parasitic & Immune-mediated DiseasesIstituto Superiore di SanitàViale Regina Elena 299Rome00161Italy

Summary

Objectives

To describe a spatio-temporal cluster of invasive meningococcal disease (IMD) due to serogroup C meningococci, occurred in a restricted area of Tuscany between January and October 2015, and the results of whole genome sequencing (WGS).

Methods

Surveillance activities and public health measures were implemented in the Region. Bacterial isolates from IMD cases were characterized by the National Reference Laboratory of the Istituto Superiore di Sanità (ISS), and WGS was performed on available strains. The kSNP software was used to identify core genome SNPs.

Results

Overall, 28 IMD cases due to meningococcus C were identified up to 31st October, 2015. Of them, 26 were due to meningococcus C:P1.5-1,10-8: F3-6:ST-11 (cc11) and 2 to C:P1.5-1,10-8: F3-6:ST-2780 (cc11). WGS of 13 meningococci isolated during the outbreak occurred in Tuscany in 2015 showed higher similarity when compared with those of 47 C: P1.5-1,10-8: F3-6:ST-11 (cc11) invasive strains from sporadic cases previously detected in Italy.

Conclusions

A highly aggressive meningococcal C strain was involved in the cluster of severe IMD occurred in Tuscany, a Region with high vaccine coverage among children. Whether this was due to low herd immunity related to the short duration of vaccine protection needs further investigation.

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Keywords : cc11, Meningococci, Neisseria meningitidis, Surveillance, Whole genome sequencing

Plan

Introduction

Materials and methods

IMD surveillance and control

Microbiological analyses

Whole genome sequencing

Genome comparison

SNPs analysis

Results

Epidemiological findings

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Vol 73 - N° 2

P. 136-144 - août 2016 Retour au numéro

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Genome-based study of a spatio-temporal cluster of invasive meningococcal disease due to Neisseria meningitidis serogroup C, clonal complex 11 - 10/07/16

Summary

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Methods

Results

Conclusions

Plan

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