To report novel vitreous body microarchitecture findings using high-resolution spectral-domain optical coherence tomography (HR-SD-OCT).

Prospective, cross-sectional study.

Horizontal and vertical retinal cross-sectional images that were 10 mm long were acquired from 17 eyes of 17 young healthy volunteers using HR-SD-OCT with enhanced vitreous imaging (EVI). Images were acquired through the fovea, upper vessel arcade, and lower vessel arcade.

Three new findings on vitreous body microarchitecture were found. First, material located between the retina and posterior vitreous cortex was easily detected in 90% of upper and lower vessel arcade scans. Most scans contained hyperreflective dots and multilayered hyperreflective lines around the detached vitreous cortex. Second, a lamellar structure was observed in the vitreous body in 70%–80% of all scans, excluding vertical scans of the upper arcade vessel area. Third, tubular zones of hypodensity were detected in >80% of scans, excluding horizontal scans of the macula. Interestingly, the location of tubular zones of hypodensity seemed to correspond with the location of retinal vessels. Subject age, refractive error, and axial length were not significantly different in scans with and without material between the retina and vitreous, lamellar structures, and tubular zones of hypodensity.

The microarchitecture of the vitreous body can be visualized using HR-SD-OCT and EVI.