High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood - 25/08/16

Doi : 10.1016/j.jpeds.2016.05.078

Changlong Guo, MD, PhD ^1,^{^{∗
Contributed equally.}}, Qidi Wang, MD, PhD ^1,^{^{∗
Contributed equally.}}, Xiaofang Cao, MD ^1,^⁎ , Ying Yang, MD, PhD ¹, Xin Liu, PhD ^1,², Lisha An, MD ¹, Ruikun Cai, PhD ¹, Meng Du, MD ^1,³, Guangyu Wang, MD ¹, Yue Qiu, MD ^1,³, Zuoqi Peng, MD ¹, Jian Han, MD, PhD ⁴, Shuhua Ni, PhD ⁵, Xuerui Tan, MD, PhD ⁵, Li Jin, MD ⁶, Song Yu, MD ⁷, Huiying Wang, MD ⁸, Chunlin Wang, PhD ^1,⁹, Xingyu Wang, MD, PhD ^1,², Xu Ma, MD, PhD ^1,³
¹ Department of Genetics, National Research Institute for Family Planning, Beijing, China
² Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China
³ Graduate School of Peking Union Medical College, Beijing, China
⁴ HudsonAlpha Institute for Biotechnology, Huntsville, AL
⁵ First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China
⁶ Beijing Friendship Hospital, Capital Medical University, Beijing, China
⁷ Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Healthy Care Hospital, Beijing, China
⁸ Beijing Shijitan Hospital, Capital Medical University, Beijing, China
⁹ Stanford Genome Technology Center, Stanford University, Palo Alto, CA

^∗Reprint requests: Ms. Xiaofang Cao, MD, Department of Genetics, National Research Institute for Family Planning, 12 Dahuisi Road, Haidian, Beijing 100081, China.Department of GeneticsNational Research Institute for Family Planning12 Dahuisi RoadHaidian, Beijing100081China

Abstract

Objective

To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels.

Study design

High-throughput sequencing was performed to analyze the expression of TRB-CDR3 and IGH-CDR3 in circulating T and B cells isolated from 20 healthy adults, 56 pregnant women, and 40 newborns.

Results

Our results revealed different immunological characteristics between newborn and adults, such as distinctive complementarity determining region 3 (CDR3) lengths, usage bias of variable and joining segments, random nucleotide addition, a large number of unique CDR3 peptides, and a greater repertoire diversity. Moreover, each newborn had a distinctive TRB-/IGH-CDR3 repertoire that was independent of the maternal immune status.

Conclusions

This study presents comprehensive, unrestricted profiles of the TRB/IGH-CDR3 repertoire of newborns, pregnant women, and healthy adults at a sequence-level resolution. Our data may contribute to a better understanding of the immune system of newborns and benefit the efficient application of umbilical cord blood transplantation in future.

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Keywords : umbilical cord blood, high-throughput sequencing, TRB-/IgH-CDR3 repertoires

Abbreviations : BM, CDR3, D, D50, HIGH, HSC, HTBI, IGH, IL, J, N addition, NGS, nt, PBMC, PCR, TRB, UCB, V

Plan

Methods

High-Throughput Sequencing

Inheritance of the TRB/IGH-CDR3 Repertoires in Newborns by Mothers

Discussion

Supported by International Science & Technology Cooperation Program of China (0102012DFB30130). The authors declare no conflicts of interest.

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Vol 176

P. 69 - septembre 2016 Retour au numéro

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High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood - 25/08/16

Abstract

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Study design

Results

Conclusions

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