Multi-spacer typing as an effective method to distinguish the clonal lineage of Clostridium butyricum strains isolated from stool samples during a series of necrotizing enterocolitis cases - 18/04/17
Summary |
Background |
Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease with high morbidity and mortality that predominantly affects preterm neonates during outbreaks. In a previous study, the present authors identified 15 Clostridium butyricum isolates from stool samples during a series of NEC cases involving four neonatal intensive care units. A clonal lineage of these strains was observed by in-silico multi-locus sequence typing.
Aim |
To confirm the previous findings by sequencing a larger number of C. butyricum genomes and using other genotyping approaches.
Methods |
The previously isolated 15 C. butyricum strains were characterized and compared with 17 other commensal and environmental C. butyricum strains using whole-genome sequencing (WGS). In addition, the clustering was analysed using multi-spacer sequence typing (MST).
Findings |
The core genome of C. butyricum was composed of 1251 genes, and its pan-genome consisted of 12,628 genes with high variability between strains. It was possible to distinguish the clonal lineage of strains from a series of NEC cases, forming three clades with geographical clustering. The results obtained using WGS and MST approaches were congruent.
Conclusion |
MST is a fast, cheap and effective genotyping method for investigating NEC outbreaks associated with C. butyricum.
Le texte complet de cet article est disponible en PDF.Keywords : Necrotizing enterocolitis, Clostridium butyricum, Whole-genome sequencing, In-silico multi-locus sequence typing, Multi-spacer sequence typing, Outbreak
Plan
Vol 95 - N° 3
P. 300-305 - mars 2017 Retour au numéro
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