Crying Time and ROR?/FOXP3 Expression in Lactobacillus reuteri DSM17938-Treated Infants with Colic: A Randomized Trial - 13/12/17
, Maria Garro, MD 1, Paola Montanari, BS 2, Ilaria Galliano, PhD 2, Massimiliano Bergallo, PhD 2Abstract |
Objectives |
To evaluate crying time, retinoid-related orphan receptor-γ (RORγ) and forkhead box P3 (FOXP3) messenger RNA levels (transcription factors that can modulate T cell responses to gut microbes), and to investigate gut microbiota and fecal calprotectin in infants treated with Lactobacillus reuteri for infantile colic.
Study design |
A double-blind, placebo-controlled randomized trial was conducted in primary care in Torino from August 1, 2015 to September 30, 2016. Patients suffering from infantile colic were randomly assigned to receive daily oral L reuteri (1 × 108 colony forming unit) or placebo for 1 month. Daily crying times were recorded in a structured diary. FOXP3 and RORγ messenger RNA in the peripheral blood was assessed with real-time TaqMan reverse transcription polymerase chain reaction. Gut microbiota and fecal calprotectin were evaluated.
Results |
After infants with colic were supplemented with L reuteri DSM 17938 for 30 days, crying times were significantly shorter among infants with colic in the probiotic group compared with infants in the placebo group (74.67 ± 25.04 [IQR = 79] minutes /day vs 147.85 [IQR = 135] minutes /day [P = .001]). The FOXP3 concentration increased significantly (P = .009), resulting in decreased RORγ/FOXP3 ratios: 0.61 (IQR = 0.60) at day 0 and 0.48 (IQR = 0.28) at day 30 (P = .028). Furthermore, the probiotic increased the percentage of Lactobacillus (P = .049) and decreased fecal calprotectin (P = .0001).
Conclusions |
Infants with colic treated with L reuteri for 30 days had a significantly decreased crying time and an increased FOXP3 concentration, resulting in a decreased RORγ/FOXP3 ratio. The treatment reduced fecal calprotectin.
Trial registration |
ClinicalTrials.gov: NCT00893711.
Le texte complet de cet article est disponible en PDF.Keywords : infantile colic, FOXP3, fecal calprotectin, gut microbiota, probiotic, RORγ, Th17/Treg balance
Abbreviations : FOXP3, IL, mRNA, PCR, RORγ, TaqMan RT-PCR, Th, Treg
Plan
| Noos srl Roma (Italy) provided support to perform the calprotectin fecal analysis. MIUR Roma (Italy) provided all material necessary for the other analyses. The authors declare no conflicts of interest. |
Vol 192
P. 171 - janvier 2018 Retour au numéro
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