An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1 - 15/12/17
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Abstract |
Background |
Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.
Methods |
Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.
Results |
The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.
Conclusion |
These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.
Le texte complet de cet article est disponible en PDF.Keywords : Snail-1, Urinary bladder cancer, Epithelial to mesenchymal transition, E-cadherin, Vimentin, MMPs
Plan
Vol 96
P. 545-550 - décembre 2017 Retour au numéro
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