Long noncoding RNA BDNF-AS is associated with clinical outcomes and has functional role in human prostate cancer - 30/04/18
, Zhongling Dou ⁎ , Shuguang We, Zhiyi Zhu, Dong Pan, Zhaohui Jia, Hui Liu, Xiaobin Wang, Guoqiang YuBienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
Background |
The underlying molecular mechanisms of prostate cancer (CaP) are largely unknown. We investigated the expression, prognostic value and functional role of long non-coding RNA (lncRNA) brain-derived neurotrophin factor antisense (BDNF-AS) in CaP.
Methods |
Clinical tumor samples were excised from patients with CaP. Their endogenous BDNF-AS expression levels were evaluated by qRT-PCR. Correlations between CaP patients’ endogenous BDNF-AS expression and their clinicopathological factors, overall survival were statistically analyzed. BDNF-AS expression levels were also probed in immortal CaP cell lines. In LNCaP and PC-3 cells, BDNF-AS was ectopically overexpressed through lentiviral transduction. The functions of BDNF-AS upregulation on CaP cell development were evaluated both in vitro and in vivo.
Results |
BDNF-AS was downregulated in human CaP tumors. Low BDNF-AS expression was correlated with CaP patients’ poor prognosis and shorter overall survival. BDNF-AS was also found to be lowly expressed in CaP cell lines. In LNCaP and PC-3 cells, lentivirus-driven BDNF-AS overexpression exerted significantly tumor-suppressing effects on hindering cancer cell proliferation and invasion in vitro, and explant growth in vivo.
Conclusion |
Downregulated BDNF-AS in CaP patients could be a potential prognostic biomarker for predicating poor prognosis and survival. Upregulating BDNF-AS may be a novel molecular intervening target for CaP treatment.
Le texte complet de cet article est disponible en PDF.Keywords : CaP, lncRNA, BDNF-AS, Cancer proliferation, Invasion, Explant
Plan
Vol 102
P. 1105-1110 - juin 2018 Retour au numéro
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