Low-level light therapy (LLLT) is used to stimulate cell function or reduce pain by applying light emitting diodes (LED) to the skin. LLLT is known to contribute to the neuronal recovery during the reconstruction of brain tissue for the treatment of degenerative disorders. This research aims to confirm the slow oscillations of cerebral hemodynamics changes when LLLT is applied to the elderly and patients with mild cognitive impairment (MCI).

Eight patients with MCI and 7 healthy elderly participated in this study. They were randomly divided into four groups; carotid artery stimulation, vertebral artery stimulation, simultaneous carotid and vertebral artery stimulation, or sham stimulation. The subjects received LLLT using Color DNA® (Color Seven Co.) with a LED light source for 30minutes a day during 20 days. The hemodynamic responses were recorded by an fNIRS system (NIRScout®) with 74 channels at the first and the 20th intervention days, before, during, and after the LLLT. The spectral power density over the very low-frequency oscillations (VLFO) of cerebral hemodynamics was calculated using Welch technique.

In both healthy and MCI patients, VLFO increased in the whole cerebral area during LLLT compared to the resting state, and these changes are more contrasting in the condition with simultaneous carotid and vertebral artery stimulation condition. These characteristics were observed in both the first and 20th intervention days however, VLFO in the 20th intervention day were more increased than the first day.

Increase of very low frequency oscillations demonstrated increasing spontaneous activity of cerebrovascular tone and neuronal activation. These findings may suggest a possibility of modulating effect of LLLT on neuronal activity and blood vessel reconstruction.