Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway - 25/08/18
, Kaiyan Sun a , Jie Chu a , Yunhui Qu a , Xue Zhao b , Huiqing Yin a , Liang Ming a , Junhu Wan a, ⁎ , Fucheng He a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Highlights |
• | FTH1P3 is significantly upregulated in esophageal squamous cell carcinoma (ESCC). |
• | The expression level of FTH1P3 is correlated with ESCC lymph node metastasis. |
• | Knockdown FTH1P3 remarkably inhibits the proliferation, migration, and invasion capacity of ESCC cells. |
• | FTH1P3 functions as an oncogene in ESCC by regulating the SP1/NF-kB signaling pathway. |
Abstract |
Aims： |
Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).
Main methods |
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays.
Key findings |
FTH1P3 was significantly upregulated in ESCC tissues and cells (P < 0.001). Knockdown of FTH1P3 notably decreased the proliferation, migration, and invasion capacity of ESCC cells. Silencing of FTH1P3 decreased the expression of specificity protein 1 (Sp1) and NF-kB (p65) in EC9706 and EC1.
Significance |
FTH1P3 plays a crucial role in ESCC tumorigenesis, and can be used as a potential therapeutic target for ESCC.
Le texte complet de cet article est disponible en PDF.Keywords : Esophageal squamous cell carcinoma, Long non-coding RNA, FTH1P3, Biological function
Plan
Vol 106
P. 1570-1577 - octobre 2018 Retour au numéro
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