Chitosan based thermoresponsive hydrogel containing graphene oxide for bone tissue repair - 20/09/18
, Selvaraj Vimalraj b, ⁎ , Dhanasekaran Anuradha bBienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
Highlights |
• | Chitosan/glycerophosphate/graphene oxide hydrogel is injectable and thermosensitive. |
• | GO addition improves protein adsorption, swelling and biodegradation properties. |
• | GO inclusion enhances exogenous biomineralization with and without cells. |
• | The hydrogel is biocompatible and promotes osteo-differentiation of MSCs. |
Abstract |
Chitosan (CS), glycerophosphate (GP) based injectable hydrogels are explored for its implications in bone defect healing and regeneration. Both acellular and cell laden CS based hydrogels are widely investigated and improved through the inclusion of various nanoparticles, polymers and bioactive molecules. In order to improve its applicability for bone tissue repair, we developed an injectable, thermosensitive CS hydrogel containing graphene oxide (GO) and investigated its properties. The hydrogels were investigated for its porous architecture using scanning electron microscopy (SEM), swelling property, protein adsorption ability, degradation rate and exogenous biomineralization. GO addition improved the physico-chemical properties with notable betterment. The CS/GP/GO hydrogel was biocompatible to mesenchymal stem cells and they were metabolically active upon encapsulation. The hydrogel promoted osteogenic differentiation of mouse mesenchymal stem cells by upregualtion of Runt-related transcription factor 2 (Runx2), Alkaline phosphatase (ALP), Type -1 collagen (COL-1), and osteocalcin (OC) under osteogenic conditions. The hydrogel proves to be an amenable platform for carrying cells and exhibited suitable properties to be a potential candidate for bone tissue regeneration.
Le texte complet de cet article est disponible en PDF.Keywords : Chitosan, Graphene oxide, Mesenchymal stem cells, Runx2, Bone
Plan
Vol 107
P. 908-917 - novembre 2018 Retour au numéro
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