Characterization of polysaccharide from Helicteres angustifolia L. and its immunomodulatory activities on macrophages RAW264.7 - 09/12/18
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 9 |
| Iconographies | 6 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Polysaccharide SPF3-1 was separated from HACP with yield of 9.8%. |
• | SPF3-1 increased RAW264.7 cell proliferation and phagocytosis capacity. |
• | SPF3-1 enhanced the NO and cytokine levels in RAW264.7 cells. |
• | SPF3-1 can be used as functional ingredients in nutraceuticals or pharmaceuticals. |
Abstract |
Helicteres angustifolia L. (H. angustifolia) has been widely used as a remedy against various types of illness relating to immune response, such as inflammations and fever. In order to characterize the structure and identify the immunomodulatory activity of polysaccharide from H. angustifolia, a polysaccharide fraction (SPF3-1) was purified from H. angustifolia by using DEAE Sepharose Fast Flow and Sephacry S-400 chromatography, successively. Physicochemical analysis demonstrated that SPF3-1 is an acidic heteropolysaccharide with a molecular weight of about 13.36 kDa; in vitro immunomodulatory assay reflects that SPF3-1 could significantly (p < 0.05) enhance the proliferation of macrophages, stimulate the macrophages phagocytic capacity, as well as induce NO and immunomodulatory cytokines generation. All the results suggest that SPF3-1 from H. angustifolia possesses potent immunomodulatory activity and could be further developed as new products for medicines or functional foods.
Le texte complet de cet article est disponible en PDF.Keywords : Helicteres angustifolia L., Immunomodulatory activity, Macrophages, Polysaccharide
Plan
Vol 109
P. 262-270 - janvier 2019 Retour au numéro
Article précédent
- Ginsenoside Rb3 protects cardiomyocytes against hypoxia/reoxygenation injury via activating the antioxidation signaling pathway of PERK/Nrf2/HMOX1
- Jing Sun, Xiaohong Yu, Haiquan Huangpu, Fengzhen Yao
- Mitochondrial dysfunction as a mechanism involved in the pathogenesis of cirrhosis-associated cholemic nephropathy
- Reza Heidari, Leila Mandegani, Vahid Ghanbarinejad, Asma Siavashpour, Mohammad Mehdi Ommati, Negar Azarpira, Asma Najibi, Hossein Niknahad
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?