Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity - 04/04/19
Abstract |
Background |
Cockroach allergens are an important cause of IgE-mediated sensitization in inner-city asthmatic patients. However, cockroach extracts used for diagnosis and immunotherapy are not standardized.
Objective |
We sought to determine the allergen content of nonstandardized German cockroach extracts and the levels of sensitization to an expanded set of cockroach allergens as determinants of in vitro extract potency for IgE reactivity.
Methods |
Twelve German cockroach extracts were compared for allergen content and potency of IgE reactivity. Bla g 1, Bla g 2, and Bla g 5 were measured by using immunoassays. IgE antibody levels to 8 purified recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11 were measured by using ImmunoCAP. IgE antibody binding inhibition assays were performed to assess extract in vitro potencies (concentration inhibiting 30% of the total IgE antibody-binding inhibition) relative to an arbitrarily selected reference extract in 5 patients with cockroach allergy.
Results |
Allergen levels were highly variable. Three new major allergens (groups 6, 9, and 11), were identified among highly cockroach-sensitized subjects (CAP class ≥ 3). Sensitization profiles were unique per subject without immunodominant allergens. The sum of IgE to 8 allergen components showed a good correlation with cockroach-specific IgE levels (r = 0.88, P < .001). In vitro potencies varied among different extracts per subject and among subjects for each extract.
Conclusions |
The in vitro potency of German cockroach extracts for IgE reactivity depends on allergen content and allergen-specific IgE titers of patients with cockroach allergy. These factors are relevant for selection of potent extracts to be used for immunotherapy and for the design and interpretation of data from immunotherapy trials.
Le texte complet de cet article est disponible en PDF.Key words : Cockroach allergy, non-standardized extracts, cockroach allergen components, immunotherapy, diagnosis
Abbreviations used : APA, IC30
Plan
| Research in this publication was supported by the National Institute of Allergy and Infectious Diseases/National Institutes of Health under Award Number R01AI077653 (to A.P. and M.D.C.), U19 AI135731 and U19 AI100275 (to A.S.), and UM1 AI114271 (Inner-City Asthma Consortium). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. |
|
| Disclosure of potential conflict of interest: J. Glesner, S. Filep, L. D. Vailes, S. Wünschmann, M. D. Chapman, and A. Pomés are employed by Indoor Biotechnologies, and M. D. Chapman is President and CEO of the company. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 143 - N° 4
P. 1474 - avril 2019 Retour au numéro
Article précédent
- Anaphylaxis after vaccination reported to the Vaccine Adverse Event Reporting System, 1990-2016
- John R. Su, Pedro L. Moro, Carmen S. Ng, Paige W. Lewis, Maria A. Said, Maria V. Cano
- Hypomorphic caspase activation and recruitment domain 11 (CARD11) mutations associated with diverse immunologic phenotypes with or without atopic disease
- Batsukh Dorjbal, Jeffrey R. Stinson, Chi A. Ma, Michael A. Weinreich, Bahar Miraghazadeh, Julia M. Hartberger, Stefanie Frey-Jakobs, Stephan Weidinger, Lena Moebus, Andre Franke, Alejandro A. Schäffer, Alla Bulashevska, Sebastian Fuchs, Stephan Ehl, Sandhya Limaye, Peter D. Arkwright, Tracy A. Briggs, Claire Langley, Claire Bethune, Andrew F. Whyte, Hana Alachkar, Sergey Nejentsev, Thomas DiMaggio, Celeste G. Nelson, Kelly D. Stone, Martha Nason, Erica H. Brittain, Andrew J. Oler, Daniel P. Veltri, T. Ronan Leahy, Niall Conlon, Maria C. Poli, Arturo Borzutzky, Jeffrey I. Cohen, Joie Davis, Michele P. Lambert, Neil Romberg, Kathleen E. Sullivan, Kenneth Paris, Alexandra F. Freeman, Laura Lucas, Shanmuganathan Chandrakasan, Sinisa Savic, Sophie Hambleton, Smita Y. Patel, Michael B. Jordan, Amy Theos, Jeffrey Lebensburger, T. Prescott Atkinson, Troy R. Torgerson, Ivan K. Chinn, Joshua D. Milner, Bodo Grimbacher, Matthew C. Cook, Andrew L. Snow
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?