Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro - 04/04/19
, Dan Zhang b, 1 , Qiuxia Cui c , Kun Wang b , Jianing Tang c , Zhisu Liu a, ⁎ , Gaosong Wu c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 8 |
| Iconographies | 3 |
| Vidéos | 0 |
| Autres | 0 |
Highlights |
• | Sapylin could promote wound healing and reduce seroma after modified radical mastectomy. |
• | After treatment with Sapylin several cytokines were increased in the drainage fluid. |
• | Wound drainage fluids in Sapylin group promote angiogenesis in vitro. |
• | Wound drainage fluids in Sapylin group promote collagen deposition in vitro. |
Abstract |
Background |
The occurrence of seroma formation and long-term wound healing remain challenging complications after modified radical mastectomy. Sapylin is a drug used to reduce seroma formation and enhance wound closure, but these results remain controversial. We aimed to investigate the potential mechanism.
Methods |
A prospective, consecutive cohort study included 120 patients diagnosed with breast cancer who underwent modified radical mastectomy was designed. Patients were randomized into two group, using or not using OK-432 (sixty patients per group) during surgeries. Patients’ drainage fluids were collected for three days after surgery. Inflammatory cytokines and chemokines were measured with ELISA assays. The proliferative, migratory, and angiogenic capacity of HUVEC and HFL1 cells HUVEC and HFL1 cells were measured after being treated with drainage fluids.
Results |
Our clinic data showed that there was no statistical significance between the two groups in patient characteristics before surgery. However, the outcomes of patients in experimental group were significantly better than those in control group. In vitro studies, the results of ELISA assays showed that several cytokines, including IL-1a, IL-6, TGF-β1, bFGF and VEGF were increased in the drainage fluids treated with Sapylin. The proliferative, migratory, and angiogenic capacity of HUVEC and HFL1 cells were significantly enhanced after being treated with Sapylin group drainage fluids.
Conclusion |
Sapylin could stimulate the body to secrete a variety of cytokines to promote wound healing by promoting endothelial cell proliferation and migration, angiogenesis and by increasing fibroblast migration and collagen deposition.
Le texte complet de cet article est disponible en PDF.Abbreviations : ELISA, IL-1, IL-6, TGF-β1, bFGF, VEGF, TNF-α, HUVECs, HFL1, FBS, Ham’s F-12 K, CCK8, RIPA, SDS-PAGE, PVDF, HRP-conjugated, α-SMA, BMI, IDCA, DCIS, TNM, WHO
Keywords : Sapylin, Breast Cancer, Wound Healing, Cytokines
Plan
Vol 113
Article 108706- mai 2019 Retour au numéro
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