Anti-biofilm, nitric oxide inhibition and wound healing potential of purpurin-18 phytyl ester isolated from Clinacanthus nutans leaves - 04/04/19
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 13 |
| Iconographies | 10 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Aims |
Clinacanthus nutans (C. nutans) has demonstrated anti-inflammatory activity, however, the active compound generating this activity remains unknown. The aim of this study was to identify the bioactive compound in C. nutans responsible for its anti-inflammatory, in-vitro wound healing, and anti-biofilm activities.
Main methods |
A pure compound was isolated from the chloroform extract (CE) of C. nutans leaves by chromatographic techniques and bioassay-guided fractionation. This compound’s structure was determined by spectroscopic analyses (FTIR/NMR/HRES-MS). Biological activities were evaluated using cytotoxicity, nitric oxide (NO), wound scratch, anti-microbial activity, and anti-biofilm assays; and the compound’s bactericidal depth into the biofilm was visualized by confocal laser scanning microscopy.
Key findings |
CE and its pure isolated compound, purpurin-18 phytyl ester (P18PE), significantly inhibited lipopolysaccharide (LPS)-induced NO production in RAW 264.7 cells at concentrations of 100 μg/ml and 10–100 μg/ml, respectively. These concentrations significantly induced wound closure by human gingival fibroblasts. CE (100–1000μg/ml) and P18PE (1–500 μg/ml) did not inhibit Streptococcus (S.) mutans growth. However, these concentrations significantly reduced S. mutans biofilm formation below 50% at 250 μg/ml for CE, and 25 μg/ml for P18PE (p<0.05).
Significance |
C. nutans contains a bioactive compound, P18PE, which exhibits anti-inflammatory, in-vitro wound healing, and anti-biofilm activities.
Le texte complet de cet article est disponible en PDF.Keywords : Clinacanthus nutans, Purpurin-18 phytyl ester, Anti-inflammation, In-vitro wound healing activity, Anti-biofilm
Plan
Vol 113
Article 108724- mai 2019 Retour au numéro
Article précédent
- Novel naphthalene-enoates: Design and anticancer activity through regulation cell autophagy
- Meng Di Yang, Xiao Bao Shen, Yang Sheng Hu, Yan Yan Chen, Xin Hua Liu
- RETRACTED: Protective effect of DMDD, isolated from the root of Averrhoa carambola L., on high glucose induced EMT in HK-2 cells by inhibiting the TLR4-BAMBI-Smad2/3 signaling pathway
- Hongliang Zhang, Xiaojie Wei, Shunyu Lu, Xing Lin, Jianchun Huang, Lixiu Chen, Xiang Huang, Luhui Jiang, Yuchun Li, Luhui Qin, Jinbin Wei, Renbin Huang
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?