Given the population-level implications of antibiotic resistance and the importance of antibiotic stewardship in containment and prevention of resistance, public health has a vested interest in strengthening antibiotic stewardship efforts. There are opportunities for public health collaboration at all levels including local health departments, state public health programs, and through federal public health entities. This article discusses existing public health stewardship activities, opportunities for collaboration between public health and key partners in antibiotic stewardship programs, the potential for improvement and expansion of current activities, and possible new modes of collaboration that could be pursued.