Plasma protein profiling reflects TH1-driven immune dysregulation in common variable immunodeficiency - 05/08/20
Abstract |
Background |
Common variable immunodeficiency (CVID) is a disorder characterized by antibody deficiency. A significant fraction of the patients suffer from immune dysregulation, which leads to increased morbidity and mortality. The pathogenesis of this condition is poorly understood.
Objective |
Our aim was to find out whether the plasma protein signature in CVID is associated with clinical characteristics and lymphocyte aberrations.
Methods |
A highly sensitive proximity extension assay was used for targeted profiling of 145 plasma proteins in 29 patients with CVID. Phenotyping of peripheral lymphocytes was done by flow cytometry. The findings were correlated with the burden of immune dysregulation.
Results |
Unsupervised clustering of plasma protein profiles identified 2 distinct groups of patients with CVID that differed significantly in terms of the degree of complications due to immune dysregulation and in terms of the frequency of activated B- and T-cell subpopulations. Pathway analysis identified IFN-γ and IL-1β as the top enriched upstream regulators associated with higher grade of immune dysregulation. In addition, CVID was found to be associated with increased plasma levels of the B-cell–attracting chemokine CXCL13.
Conclusion |
Clustering based on plasma protein profiles delineated a subgroup of patients with CVID with activated T cells and clinical complications due to immune dysregulation. Thus, data indicate that CVID-associated immune dysregulation is a TH1-mediated inflammatory process driven by the IFN-γ pathway.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : CVID, immunodeficiency, lymphoproliferation, immune dysregulation, proximity extension immunoassay, plasma proteomics
Abbreviations used : AUC, CRTAM, CVID, CXCL, FcRL6, HLA-DR, IPA, NF-κB, Pcorr, PCA, PEA, PDCD1, TNFRSF
Plan
| Supported by ALF-grants, Region Östergötland, Sweden. |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 146 - N° 2
P. 417-428 - août 2020 Retour au numéro
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