Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions - 19/03/21
, P. Novak b , B. Nowak c , A. Furgała a, K. Wójcik-Piotrowicz d , P. Piszczek a, ⁎ , P. Guzdek e , J. Pytko-Polończyk f Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
The aim of the current study was to investigate the influence of low-frequency electromagnetic field (LF-EMF) exposure on viability parameters of oral mucosa keratinocytes cultured in in vitro conditions. The effect of LF-EMF stimulation on cell viability was also specified in the simultaneous presence of lipopolysaccharide (LPS) infectious agent or minocycline (Mino) anti-inflammatory agent. Viability parameters such as early-, late apoptosis and necrosis of keratinocytes were analysed by the flow cytometry method (FCM).
The exposure of human oral keratinocyte cell cultures to LF-EMF acting alone or combined with LPS/minocycline agents caused changes in the percentage of cells that undergo programmed or incidental cell death. The overall obtained results are compiled in a graphical form presented in Fig. 1.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Apoptosis and necrosis in keratinocytes is influenced by exposure to LF-EMF. |
• | LF-EMF and/or LPS/minocycline treatment changes keratinocytes viability. |
• | LF-EMF possibly modulates an anti-inflammatory activities of keratinocytes. |
• | LF-EMF might play therapeutic role in healing process of oral mucosa inflammation. |
Keywords : Electromagnetic field, Oral keratinocytes, Cell viability, Lipopolysaccharide, Minocycline
Plan
Vol 137
Article 111340- mai 2021 Retour au numéro
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