The present study reports the cytoprotective efficacy of vitamin C, E and beta-carotene against chromium (VI) induced oxidative stress in murine macrophages. Addition of chromium (VI) resulted in enhanced cytotoxicity as revealed by fall in neutral red uptake and increase in LDH release compared to control cells. Further there was an appreciable increase in apoptosis, ROS production and fall in reduced glutathione (GSH), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities. Chromium also inhibited macrophage proliferation and phagocytic activity. Addition of vitamin C but not vitamin E and beta-carotene inhibited chromium induced cytotoxicity, ROS generation and apoptosis. Vitamin C significantly inhibited NO production, enhanced macrophage proliferation and phagocytic activity while vitamin E and beta-carotene had marginal effect.