Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula - 12/12/21
, Ya-Nan Yang b, 1 , Ying Liang a , Rui Lang a , Qin Zeng c , Lei Yan d , Ren-Huan Yu a, ⁎ , Chong-Ming Wu b, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 12 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Background |
Jianpi-Qushi-Heluo formula (JQHF) has been used to treat idiopathic membranous nephropathy (IMN) in hospitals for many years.
Purpose |
Elucidating the protective effect and exploring the potential mechanism of JQHF against IMN.
Methods |
Passive Heymann nephritis (PHN) was induced in rats by a single tail vein injection of anti-Fx1A antiserum. Then, the animals were treated with JQHF at 16.2 g/kg or 32.4 g/kg, with benzepril (10 mg/kg) as a positive control. Renal function was evaluated by biochemical measurements and pathological testing. Fecal samples were collected before and after treatment to analyze the gut microbiota composition by shotgun whole metagenome sequencing.
Results |
JQHF exhibited potent efficacy in ameliorating PHN at both doses, as revealed by decreasing the deposition of IgG and C5b-9, relieving podocyte injury, and reducing glomerular and tubular cell apoptosis. The lower dose was corresponding to the clinical dosage and showed better therapeutic effects than the higher dose. Metagenomic analysis showed that gavage with 16.2 g/kg of JQHF shifted the structure of the gut microbiota in PHN rats and significantly increased the relative abundances of Prevotella copri, Lactobacillus vaginalis and Subdoligranulum variabile. Particularly, S. variabile was strongly negatively correlated with serum levels of TC and TG, the deposition of IgG and C5b-9, and apoptosis of glomerular cells.
Conclusions |
The JQHF is an effective agent for the treatment of experimental PHN. The PHN-allevating effect of JQHF is associated with specific alternation of gut microbiota.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | IMN is the leading form of idiopathic nephrotic syndrome in adults. |
• | The curative effect of JQHF is obvious for the treatment of IMN without any side effect. |
• | JQHF is effective in ameliorating of proteinuria and reduce the renal pathological damage of experimental PHN rats. |
• | Enrichment of L. vaginalis and S. variabile may partially account for the therapeutic effect of JQHF. |
Keywords : Idiopathic membranous nephropathy (IMN), Passive Heymann nephritis (PHN), Jianpi Qushi Heluo formula (JQHF), Gut microbiota, Subdoligranulum variabile
Plan
Vol 145
Article 112475- janvier 2022 Retour au numéro
Article précédent
- Melatonin pre-treated bone marrow derived-mesenchymal stem cells prompt wound healing in rat models
- Aljohara M. Al-Otaibi, Asma S. Al-Gebaly, Rafa Almeer, Gadah Albasher, Wedad S. Al-Qahtani, Ahmed E. Abdel Moneim
- Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
- Elsa M. Materón, Flavio M. Shimizu, Kevin Figueiredo dos Santos, Gustavo F. Nascimento, Vananélia P.N. Geraldo, Osvaldo N. Oliveira Jr, Ronaldo C. Faria
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?