Genistein alleviates renin-angiotensin system mediated vascular and kidney alterations in renovascular hypertensive rats - 16/01/22
, Putcharawipa Maneesai a , Prapassorn Potue a , Sarawoot Bunbupha b , Terdthai Tong-Un a , Wannapa Settheetham-Ishida a , Juthamas Khamseekaew a , Poungrat Pakdeechote a, c, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 13 |
| Iconographies | 9 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Genistein is a bioflavonoid mainly found in soybean. This study evaluated the effect of genistein on vascular dysfunction and kidney damage in two-kidney, one-clipped (2K1C) hypertensive rats. Male Sprague–Dawley-2K1C hypertensive rats were treated with genistein (40 or 80 mg/kg) or losartan 10 mg/kg (n = 8/group). Genistein reduced blood pressure, attenuated the increase in sympathetic nerve-mediated contractile response and endothelial dysfunction in the mesenteric vascular beds and aorta of 2K1C rats. Increases in the intensity of tyrosine hydroxylase (TH) in the mesentery and plasma norepinephrine (NE) were alleviated in the genistein-treated group. Genistein also improved renal dysfunction, hypertrophy of the non-clipped kidney (NCK) and atrophy of the clipped kidney (CK) in 2K1C rats. Upregulation of angiotensin II receptor type I (AT1R), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit 4 (Nox4) and Bcl2-associated X protein (BAX) and downregulation of B-cell lymphoma 2 (Bcl2) protein found in CK were restored by genistein. It also suppressed the overexpression of AT1R, transforming growth factor beta I (TGF-β1), smad2/3 and p-smad3 in NCK. Genistein reduced serum angiotensin converting enzyme (ACE) activity and plasma angiotensin II (Ang II) in 2K1C rats. Low levels of catalase activity as well as high levels of superoxide generation and malondialdehyde (MDA) in 2K1C rats were restored by genistein treatment. In conclusion, genistein suppressed renin-angiotensin system-mediated sympathetic activation and oxidative stress in 2K1C rats. It alleviated renal atrophy in CK via modulation of AT1R/NADPH oxidase/Bcl-2/BAX pathways and hypertrophy in NCK via AT1R/TGF-β1/smad-dependent signalling pathways.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Genistein suppresses renin-angiotensin system activation in 2K1C rats. |
• | Sympathoexcitation and endothelial dysfunction are alleviated by genistein. |
• | Decreased kidney function in 2K1C rats is improved by genistein. |
• | Genistein alleviates abnormalities renal morphology in 2K1C rats. |
Keywords : Genistein, Renin angiotensin system, Oxidative stress, Vascular dysfunction, Kidney damage, Renovascular hypertension
Plan
Vol 146
Article 112601- février 2022 Retour au numéro
Article précédent
- Changes in the expression of membrane type-matrix metalloproteinases genes (MMP14, MMP15, MMP16, MMP24) during treatment and their potential impact on the survival of patients with non-small cell lung cancer (NSCLC)
- Jacek Pietrzak, Dagmara Szmajda-Krygier, Agnieszka Wosiak, Rafał Świechowski, Katarzyna Michalska, Marek Mirowski, Marta Żebrowska-Nawrocka, Mariusz Łochowski, Ewa Balcerczak
- Epigenetic changes in inflammatory genes and the protective effect of cooked rhubarb on pancreatic tissue of rats with chronic alcohol exposure
- Xianlin Zhao, Shifeng Zhu, Juan Li, Dan Long, Meihua Wan, Wenfu Tang
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?