Controlling mechanisms of appetite and food intake is an important issue for a variety of different types of athletes. Previous studies showed omega-3 fatty acid increased appetite in patients, but results in healthy individuals are inconclusive. This study assessed the effect of omega-3 fatty acid supplements on food intake and appetite in young male athletes with normal body fat percentage. In a three-week double-blind placebo-controlled RCT in Tabriz, Iran (2019), 72 male athletes, age 22.2 (±2.5) y with 13.9 (8.5) body fat percent were randomized to either an omega-3 (2000mg/day; EPA: 360mg, DHA: 240mg) or placebo (2000mg/day paraffin) group. 3-day food diaries were completed before and after the intervention. Body composition was measured by bioelectrical impedance analysis. Appetite was assessed by visual analog scale (VAS) before and after each week of intervention. Results showed carbohydrate intake increased in the omega-3 group (MD=65.8g; 95% CI=9.1, 70.1; P=0.03). VAS score for satiety decreased. Hunger sensation, desire to eat, and desire to eat sweet foods increased in each of the three weeks. In conclusion, our findings showed that omega-3 supplementation increased appetite in healthy athletes without significant change in body fat percentage. Further research is needed to generalize the results to other populations.